Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,113	A→G	I364T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,113	0	A	G	100.0%	92.2 / NA	26	I364T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/16);  total (10/16)

ACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACG  >  NZ_CP009273/250984‑251240                                                                                                                                  |                                                                                                                                aCCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGt                                                                                                                        >  1:260106/1‑139 (MQ=255) aCCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGt                                                                                                                        >  2:221682/1‑139 (MQ=255)                        ccagccagGATATGCGCCTTTCGCTTCGGTTTGCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTgtcag                                                                                                   <  2:408365/137‑1 (MQ=255)                                     gcgcCTTTTGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCacac                                                                                    >  2:114224/1‑139 (MQ=255)                                     gcgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCacac                                                                                    >  1:117406/1‑139 (MQ=255)                                      cgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACAcc                                                                                   <  1:221682/139‑1 (MQ=255)                                       gcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCg                                                                                  <  1:114224/139‑1 (MQ=255)                                                  cGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAggg                                                                        <  1:178397/138‑1 (MQ=255)                                                  cGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAgg                                                                         <  1:313326/137‑1 (MQ=255)                                                            gccagccaGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAAcc                                                             <  2:516747/139‑1 (MQ=255)                                                                            aGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt                                             <  1:458024/139‑1 (MQ=255)                                                                                    cAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt                                      <  1:507740/138‑1 (MQ=255)                                                                                        aTGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGaa                                 >  1:35582/1‑139 (MQ=255)                                                                                           cTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca                              <  2:214188/139‑1 (MQ=255)                                                                                              accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcaca                            <  2:152782/138‑1 (MQ=255)                                                                                              accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcaca                            <  2:347682/138‑1 (MQ=255)                                                                                               ccacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACca                                                            >  1:405694/1‑105 (MQ=255)                                                                                               ccacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACca                                                            <  2:405694/105‑1 (MQ=255)                                                                                                     aGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgg                    >  1:54967/1‑139 (MQ=255)                                                                                                       tCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgggg                  <  2:42105/139‑1 (MQ=255)                                                                                                           tACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGc              >  1:81593/1‑139 (MQ=255)                                                                                                                 tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcag        >  1:312199/1‑139 (MQ=255)                                                                                                                     gATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcagcag     <  1:55460/138‑1 (MQ=255)                                                                                                                     gATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcagcag     <  2:54967/138‑1 (MQ=255)                                                                                                                       tCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACg  <  2:30564/139‑1 (MQ=255)                                                                                                                        cAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACg  >  2:260782/1‑138 (MQ=255)                                                                                                                                  |                                                                                                                                ACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACG  >  NZ_CP009273/250984‑251240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑