Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,037,050	T→G	intergenic (‑12/‑82)	BW25113_RS10320 ← / → mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,037,050	0	T	G	100.0%	133.2 / NA	37	intergenic (‑12/‑82)	BW25113_RS10320/mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/22);  total (15/22)

ATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCC  >  NZ_CP009273/2036932‑2037184                                                                                                                       |                                                                                                                                       aTAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAAttt                                                                                                                    <  2:388808/139‑1 (MQ=255)    aGTCATTGATAAAGTGGCGGAGAGAGGGGGAGTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTGCAGATGGTGGCCATGATGCCAGGAAATTTgg                                                                                                                  <  1:489298/138‑1 (MQ=255)            aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  1:524490/138‑1 (MQ=255)            aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  2:570009/138‑1 (MQ=255)            aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  1:519224/138‑1 (MQ=255)            aTAAAGGGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  2:188359/138‑1 (MQ=255)                aGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCatg                                                                                                                                  >  2:144531/1‑110 (MQ=255)                aGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCatg                                                                                                                                  <  1:144531/110‑1 (MQ=255)                aGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTc                                                                                                     >  2:53822/1‑139 (MQ=255)                      ggAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTc                                                                                               >  2:39352/1‑139 (MQ=255)                          agagGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTg                                                                                           >  1:432225/1‑139 (MQ=255)                            agGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGc                                                                                          >  1:373432/1‑138 (MQ=255)                              ggggATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAAt                                                                                       >  1:496051/1‑139 (MQ=255)                                   tttGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGt                                                                                  >  2:393150/1‑139 (MQ=255)                                          ccccGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTg                                                                           <  2:513558/139‑1 (MQ=255)                                                     ttGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGc                                                                 >  2:285220/1‑138 (MQ=255)                                                        ccccTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGc                                                             <  1:285220/139‑1 (MQ=255)                                                         cccTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGc                                                             >  2:102464/1‑138 (MQ=255)                                                                   gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  1:411126/83‑1 (MQ=255)                                                                   gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          <  2:495070/83‑1 (MQ=255)                                                                   gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          >  2:411126/1‑83 (MQ=255)                                                                   gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                          >  1:495070/1‑83 (MQ=255)                                                                      ttCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGAtt                                                 >  1:106854/1‑137 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  2:228428/138‑1 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  1:261750/138‑1 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  1:47630/138‑1 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  2:496051/138‑1 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  1:40474/138‑1 (MQ=255)                                                                        cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                              <  2:55414/138‑1 (MQ=255)                                                                         gagaCCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTg                                            <  2:432225/139‑1 (MQ=255)                                                                                gTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTg                                      >  1:158079/1‑138 (MQ=255)                                                                                 tCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                                    <  2:524703/139‑1 (MQ=255)                                                                                 tCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                                    <  1:301535/139‑1 (MQ=255)                                                                                        aGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACa                             <  1:393150/139‑1 (MQ=255)                                                                                               ccGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAAt                        >  1:517281/1‑137 (MQ=255)                                                                                                cGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCa                      <  1:102464/138‑1 (MQ=255)                                                                                                       tctcCGTTCAGATGGTGGCCATGATTCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATc               >  2:331418/1‑138 (MQ=255)                                                                                                                   tggtggCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTcc  >  1:468384/1‑139 (MQ=255)                                                                                                                       |                                                                                                                                       ATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCC  >  NZ_CP009273/2036932‑2037184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑