Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,066,165	A→G	G382G (GGA→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,066,165	0	A	G	100.0%	76.5 / NA	22	G382G (GGA→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

AAGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCC  >  NZ_CP009273/2066029‑2066300                                                                                                                                         |                                                                                                                                        aaGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGgaa                                                                                                                                       <  1:105485/139‑1 (MQ=255)    cTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATgg                                                                                                                                    <  1:368396/139‑1 (MQ=255)       aTAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCgg                                                                                                                                 >  1:165982/1‑139 (MQ=255)                 aCTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTActgctg                                                                                                                       >  1:405269/1‑139 (MQ=255)                             cGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGt                                                                                                           <  1:475057/139‑1 (MQ=255)                                                               aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGt                                                                                               >  2:68623/1‑117 (MQ=255)                                                               aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGt                                                                                               <  1:68623/117‑1 (MQ=255)                                                                   aTACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCa                                                                       <  2:372821/137‑1 (MQ=255)                                                                    tACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTc                                                                    <  1:490941/139‑1 (MQ=255)                                                                    tACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTc                                                                    <  2:365945/139‑1 (MQ=255)                                                                          cgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATc                                                              >  1:141013/1‑139 (MQ=255)                                                                          cgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTATACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATc                                                              >  2:36902/1‑139 (MQ=255)                                                                           gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   >  1:488349/1‑101 (MQ=255)                                                                           gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   <  2:488349/101‑1 (MQ=255)                                                                                   tgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt                                                     <  1:327642/139‑1 (MQ=255)                                                                                   tgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt                                                     <  1:317531/139‑1 (MQ=255)                                                                                    ggCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt                                                     >  1:166191/1‑138 (MQ=255)                                                                                     gCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCa                                                   <  2:433176/139‑1 (MQ=255)                                                                                                                   ccGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGCTGCCCTGATGCTGGAAAAAGGCAg                     >  2:44497/1‑139 (MQ=255)                                                                                                                           accGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcattca             <  1:123101/139‑1 (MQ=255)                                                                                                                               tATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCAtt                                                                     >  1:31191/1‑79 (MQ=255)                                                                                                                               tATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCAtt                                                                     <  2:31191/79‑1 (MQ=255)                                                                                                                                   cATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGaa      <  2:405269/138‑1 (MQ=255)                                                                                                                                      ggggAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGcc  >  1:77247/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        AAGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCC  >  NZ_CP009273/2066029‑2066300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑