Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,115,706	A→G	F252S (TTC→TCC)	cpsG ←	colanic acid biosynthesis phosphomannomutase CpsG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,115,706	0	A	G	100.0%	101.7 / NA	29	F252S (TTC→TCC)	cpsG	colanic acid biosynthesis phosphomannomutase CpsG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

GGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGAAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGAAATTGCCGT  >  NZ_CP009273/2115591‑2115832                                                                                                                    |                                                                                                                               ggAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCa                                                                                                         <  2:17694/139‑1 (MQ=255)       aCGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCa                                                                                                   <  1:215187/139‑1 (MQ=255)                 tggatGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCatat                                                                                         >  2:428930/1‑139 (MQ=255)                 tggatGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCatat                                                                                         >  1:67101/1‑139 (MQ=255)                            cgcgCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   <  2:32228/134‑1 (MQ=255)                            cgcgCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   <  2:226142/134‑1 (MQ=255)                            cgcgCCGGGATGTTTTTCGAGGAATGCTTCTGCCAACAGGCGGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   <  2:457310/134‑1 (MQ=255)                                 cGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTtgat                                                                         <  1:559833/139‑1 (MQ=255)                                  gggATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   >  1:393901/1‑128 (MQ=255)                                  gggATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   <  2:393901/128‑1 (MQ=255)                                                            ccaccaGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   >  2:168938/1‑102 (MQ=255)                                                            ccAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCgcgc                                                                                   <  1:168938/102‑1 (MQ=255)                                                            ccAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGc                                               <  2:420987/138‑1 (MQ=255)                                                            ccAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCa                                              >  2:187981/1‑139 (MQ=255)                                                                   gCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCgg                                        >  2:374303/1‑138 (MQ=255)                                                                      gACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTg                                                               >  2:150762/1‑112 (MQ=255)                                                                      gACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTg                                                               <  1:150762/112‑1 (MQ=255)                                                                        cAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCagtag                                  <  1:428930/139‑1 (MQ=255)                                                                        cAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCagtag                                  <  1:374303/139‑1 (MQ=255)                                                                             tagtagCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGt                             <  2:316664/139‑1 (MQ=255)                                                                               gtagCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGtt                            <  1:187981/138‑1 (MQ=255)                                                                                     cTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATa                     >  1:97649/1‑139 (MQ=255)                                                                                     cTCAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATa                     >  1:42476/1‑139 (MQ=255)                                                                                       cAATAAACTGCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATAcc                   >  2:333936/1‑139 (MQ=255)                                                                                                gCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGa           >  1:455822/1‑138 (MQ=255)                                                                                                gCCCTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGa           >  2:535426/1‑138 (MQ=255)                                                                                                  ccTTTTTCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGAAAt        >  1:549446/1‑139 (MQ=255)                                                                                                      tttCGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGAAATTg      <  2:549446/137‑1 (MQ=255)                                                                                                         cGTCAAACAGGGAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGAAATTGCCGt  <  1:70988/138‑1 (MQ=255)                                                                                                                    |                                                                                                                               GGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCGAGGAATGCTTCTGCCAACAGGCCGACAATGTAGTAGCCCTCAATAAACTGCCCTTTTTCGTCAAACAGGAAACAGCGGTCAAAATCGCCATCAAAAGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGGCAGTAGTGGGTTAGGAATACCGTTGGGGAAATTGCCGT  >  NZ_CP009273/2115591‑2115832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑