Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,135,155	T→C	N181S (AAC→AGC)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,135,155	0	T	C	100.0%	52.2 / NA	16	N181S (AAC→AGC)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/9);  total (7/9)

CCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAG  >  NZ_CP009273/2135040‑2135280                                                                                                                    |                                                                                                                              cccGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCAcgg                                                                                                        >  1:26385/1‑139 (MQ=255)     gCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGt                                                                                                    >  2:361394/1‑139 (MQ=255)                                 gCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAg                                                                        <  2:188315/139‑1 (MQ=255)                                            aaTCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGc                                                             >  1:284689/1‑139 (MQ=255)                                             aTCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCt                                                            <  2:184394/139‑1 (MQ=255)                                                gTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAg                                                         >  1:31888/1‑139 (MQ=255)                                                  ctcATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAgcg                                                       <  2:372950/139‑1 (MQ=255)                                                     aTGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTTTTAGGTCGCACCCCCCGGCCGGAAACCCCCTCAAAGCGCAGCGCAc                                                    >  2:97687/1‑139 (MQ=255)                                                                    ggCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGccc                                      <  1:182009/138‑1 (MQ=255)                                                                     gCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAACTCAGCGCCCCAATTAAAATGCCCgg                                    >  1:388303/1‑139 (MQ=255)                                                                                    tATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAACGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCgg                     >  1:194670/1‑139 (MQ=255)                                                                                     aTCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCgg                     <  2:246115/138‑1 (MQ=255)                                                                                                       cgcgCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAg  <  2:194670/139‑1 (MQ=255)                                                                                                       cgcgCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAg  <  2:284689/139‑1 (MQ=255)                                                                                                       cgcgCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAg  <  2:31888/139‑1 (MQ=255)                                                                                                       cgcgCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGCACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAg  <  1:203828/139‑1 (MQ=255)                                                                                                                    |                                                                                                                              CCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAG  >  NZ_CP009273/2135040‑2135280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑