Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,136,211	A→G	D73D (GAT→GAC)	udk ←	uridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,136,211	0	A	G	100.0%	76.7 / NA	22	D73D (GAT→GAC)	udk	uridine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/11);  total (11/11)

CGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGAT  >  NZ_CP009273/2136078‑2136344                                                                                                                                      |                                                                                                                                      cGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCa                                                                                                                                   <  1:230920/138‑1 (MQ=255)            aCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGa                                                                                                                        <  2:120936/138‑1 (MQ=255)                 cttcGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCg                                                                                                                  >  1:104396/1‑139 (MQ=255)                          aaCCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTc                                                                                                         >  1:168970/1‑139 (MQ=255)                                             aTACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGt                                                                                                              >  2:285885/1‑115 (MQ=255)                                             aTACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGt                                                                                                              <  1:285885/115‑1 (MQ=255)                                                  cgTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGa                                                                                 <  2:104396/139‑1 (MQ=255)                                                  cgTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGCTCTTCCATCGa                                                                                     >  1:298114/1‑135 (MQ=255)                                                    tATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATg                                                                               >  2:89533/1‑139 (MQ=255)                                                                  cTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGTCAGATGGCTTTGATCTTTg                                                                  >  1:360936/1‑138 (MQ=255)                                                                  cTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTg                                                                  >  1:523301/1‑138 (MQ=255)                                                                     tAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTaa                                                               >  1:559175/1‑138 (MQ=255)                                                                         ccGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCa                                                          <  2:565900/139‑1 (MQ=255)                                                                            gCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGTAGTc                                                       >  1:185586/1‑139 (MQ=255)                                                                             cAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCt                                                      >  1:550335/1‑139 (MQ=255)                                                                             cAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCt                                                      >  1:140363/1‑139 (MQ=255)                                                                                    aTTGCCGATCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTTTGGTCCATCGCGCTGGGATGGTCGTATTTGGTCTTAACGCTTTCTTCCATCGACCGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGaa                                               <  2:7759/139‑1 (MQ=255)                                                                                    aTTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGaa                                               <  1:89533/139‑1 (MQ=255)                                                                                         cGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGCTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACg                                          <  2:298114/139‑1 (MQ=255)                                                                                            gCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCg                                       <  2:550335/139‑1 (MQ=255)                                                                                                                             cagACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCa      <  2:559175/139‑1 (MQ=255)                                                                                                                                 cTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGAt  <  1:103992/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGAT  >  NZ_CP009273/2136078‑2136344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑