Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,179,484	A→G	E30G (GAA→GGA)	rcnA →	nickel/cobalt efflux protein RcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,179,484	0	A	G	100.0%	70.7 / NA	21	E30G (GAA→GGA)	rcnA	nickel/cobalt efflux protein RcnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

GGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCTTTGGCG  >  NZ_CP009273/2179349‑2179615                                                                                                                                        |                                                                                                                                    ggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGAc                                                                                                                                   >  1:37517/1‑138 (MQ=255) ggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGAc                                                                                                                                   >  2:374790/1‑138 (MQ=255)         tAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACt                                                                                                                          >  2:446435/1‑139 (MQ=255)                 gATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAAc                                                                                                                   >  1:84242/1‑138 (MQ=255)                                                   aCCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                                  >  1:259780/1‑137 (MQ=255)                                                   aCCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                                  <  2:541392/137‑1 (MQ=255)                                                   aCCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                                  >  1:541392/1‑137 (MQ=255)                                                   aCCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                                  <  2:259780/137‑1 (MQ=255)                                                            aCAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTaaa                                                                        >  2:300168/1‑138 (MQ=255)                                                                   ttcttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGt                                                                >  1:418400/1‑139 (MQ=255)                                                                     cttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGt                                                                <  1:300168/137‑1 (MQ=255)                                                                        cAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGc                                                           >  1:95832/1‑139 (MQ=255)                                                                             aGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGa                                                      <  2:189655/139‑1 (MQ=255)                                                                                         gTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACGCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCaa                                            <  2:95832/137‑1 (MQ=255)                                                                                              tcATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTc                                     >  2:195463/1‑139 (MQ=255)                                                                                                        cgcCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCa                           <  1:300121/139‑1 (MQ=255)                                                                                                                       tGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAAtt            >  2:403247/1‑139 (MQ=255)                                                                                                                       tGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGGGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGGCTGGTTAAtt            >  2:327870/1‑139 (MQ=255)                                                                                                                              cATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCtt       <  2:262315/137‑1 (MQ=255)                                                                                                                                  gCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCTTTGGCg  <  1:474556/138‑1 (MQ=255)                                                                                                                                  gCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCTTTGGCg  <  1:403247/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCTTTGGCG  >  NZ_CP009273/2179349‑2179615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑