Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,184,600	A→C	G160G (GGT→GGG)	yehC ←	fimbria/pilus periplasmic chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,184,600	0	A	C	100.0%	64.2 / NA	18	G160G (GGT→GGG)	yehC	fimbria/pilus periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/7);  total (11/7)

TTACTTTTGACATTAACACTCTGACTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTG  >  NZ_CP009273/2184472‑2184712                                                                                                                                 |                                                                                                                 ttaCTTTTGACATTAACACTCTGACTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCAc                                                                                                        >  1:395591/1‑139 (MQ=255)       ttGACATTAACACTCTGACTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGAt                                                                                                  >  2:192294/1‑139 (MQ=255)                    tctGACTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAAttt                                                                                     >  1:424296/1‑139 (MQ=255)                         cTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTaa                                                                                <  2:40337/139‑1 (MQ=255)                                           ataataGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTAcc                                                               <  2:424296/138‑1 (MQ=255)                                               taGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCt                                                                        >  2:542332/1‑125 (MQ=255)                                               taGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCt                                                                        <  1:542332/125‑1 (MQ=255)                                                  tttCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCaa                                                        >  2:265983/1‑138 (MQ=255)                                                    tCATAATTGACTTTGACCTTATTAGCTTTGACATCCGAAATCGTCAACCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAACTGTCGCTTTATTTACCGGAGCAATAc                                                     >  2:306242/1‑139 (MQ=255)                                                    tCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATAc                                                     >  2:549035/1‑139 (MQ=255)                                                    tCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATAc                                                     >  1:289337/1‑139 (MQ=255)                                                             aCTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGcc                                            >  2:250594/1‑139 (MQ=255)                                                             aCTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGcc                                            <  2:289337/139‑1 (MQ=255)                                                                       tattaGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAaacaa                                  >  1:510733/1‑139 (MQ=255)                                                                         ttaGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACt                                <  1:250594/139‑1 (MQ=255)                                                                                 gACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCg                                                                          >  2:44635/1‑89 (MQ=255)                                                                                 gACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCg                                                                          <  1:44635/89‑1 (MQ=255)                                                                                                       ttAGCTGAGTCATTTTTTATCACCAACCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTg  <  1:265983/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                 TTACTTTTGACATTAACACTCTGACTTTCTAAGGGGGCAATCATAATAGTTTCATAATTGACTTTGACATTATTAGCTTTGACATCCGAAATCGTCACCCAATTAGCTGAGTCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTG  >  NZ_CP009273/2184472‑2184712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑