Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	253,379	T→C	G122G (GGT→GGC)	frsA →	esterase FrsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	253,379	0	T	C	100.0%	110.1 / NA	32	G122G (GGT→GGC)	frsA	esterase FrsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

CCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCAC  >  NZ_CP009273/253255‑253513                                                                                                                             |                                                                                                                                       ccGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCa                                                                                                                          <  1:372800/139‑1 (MQ=255)     cgaTGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCg                                                                                                                      >  1:326430/1‑139 (MQ=255)      gatgatGTAGACGAGACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGGGTGGCAACAAAAAGCCTGGGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCgg                                                                                                                     <  2:210434/139‑1 (MQ=255)          atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                                  <  2:24072/138‑1 (MQ=255)           tttATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                                                                <  2:224056/139‑1 (MQ=255)                    tacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTacaa                                                                                                        >  2:472774/1‑138 (MQ=255)                    tacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTacaa                                                                                                        >  1:503242/1‑138 (MQ=255)                     acgGTGTTTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTacaaca                                                                                                      >  1:379317/1‑139 (MQ=255)                                        gcggcAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                                                        <  2:3532/134‑1 (MQ=255)                                        gcggcAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                                                        >  1:3532/1‑134 (MQ=255)                                                            tGGGCAACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCg                                                               >  2:42808/1‑139 (MQ=255)                                                              ggCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTAACCTCATCTGAAAGGAGATGACCTGGCCGAg                                                             >  2:457539/1‑139 (MQ=255)                                                                      aGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgccgc                                                                                               <  1:376828/97‑1 (MQ=255)                                                                      aGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgccgc                                                                                               >  2:376828/1‑97 (MQ=255)                                                                        gCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAgg                                                   <  1:472774/139‑1 (MQ=255)                                                                        gCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTAACCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAgg                                                   <  1:457539/139‑1 (MQ=255)                                                                             ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                              >  1:310309/1‑139 (MQ=255)                                                                              gtgtGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCgg                                                                                                                     <  1:345335/67‑1 (MQ=255)                                                                              gtgtGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCgg                                                                                                                     >  2:345335/1‑67 (MQ=255)                                                                              gtgtGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAg                                                             <  1:114300/123‑1 (MQ=255)                                                                              gtgtGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAg                                                             >  2:114300/1‑123 (MQ=255)                                                                                  ggCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                                  >  2:131056/1‑66 (MQ=255)                                                                                  ggCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                                  <  1:131056/66‑1 (MQ=255)                                                                                                  tgCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAagag                         >  2:448464/1‑139 (MQ=255)                                                                                                    cGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                       >  1:556276/1‑139 (MQ=255)                                                                                                              tCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGt             >  1:520089/1‑139 (MQ=255)                                                                                                              tCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGt             <  2:531539/139‑1 (MQ=255)                                                                                                                          tGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                                      <  2:383158/102‑1 (MQ=255)                                                                                                                          tGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                                      >  1:383158/1‑102 (MQ=255)                                                                                                                          tGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCt                                 <  2:218574/107‑1 (MQ=255)                                                                                                                          tGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCt                                 >  1:218574/1‑107 (MQ=255)                                                                                                                          tGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCAc  >  1:372925/1‑138 (MQ=255)                                                                                                                             |                                                                                                                                       CCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCAC  >  NZ_CP009273/253255‑253513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑