Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,268,854	A→G	S35G (AGT→GGT)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,268,854	0	A	G	100.0%	52.5 / NA	16	S35G (AGT→GGT)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

GTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGAT  >  NZ_CP009273/2268720‑2268982                                                                                                                                       |                                                                                                                                 gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                              <  1:174868/139‑1 (MQ=255)                                    tCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTg                                                                                            <  1:259875/138‑1 (MQ=255)                                       cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                              >  2:85261/1‑101 (MQ=255)                                       cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                              <  1:85261/101‑1 (MQ=255)                                       cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTgg                                                                                        >  1:48180/1‑139 (MQ=255)                                             aGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGt                                                                                   >  2:431022/1‑138 (MQ=255)                                                    tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACgg                                                                           >  1:208978/1‑139 (MQ=255)                                                                      cgcgTTTTCGTCATAACCGTCGCGGCTACTGGTCGTCATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGt                                                          >  1:164207/1‑138 (MQ=255)                                                                              cGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGaaa                                                  <  2:48180/138‑1 (MQ=255)                                                                                         tCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGa                                       >  2:508277/1‑138 (MQ=255)                                                                                                      tCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTggcg                         <  1:543917/139‑1 (MQ=255)                                                                                                      tCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTggcg                         <  2:164207/139‑1 (MQ=255)                                                                                                         ttATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGc                                                                                                            >  1:110058/1‑53 (MQ=255)                                                                                                         ttATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGc                                                                                                            <  2:110058/53‑1 (MQ=255)                                                                                                                         tCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGc      >  1:112890/1‑139 (MQ=255)                                                                                                                             gtttggtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGa   <  1:431022/138‑1 (MQ=255)                                                                                                                              tttggtttggGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTg    >  2:111178/1‑136 (MQ=255)                                                                                                                              tttggtttggGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTg    <  1:111178/136‑1 (MQ=255)                                                                                                                              tttggtttggGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGAt  >  1:153484/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                 GTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGAT  >  NZ_CP009273/2268720‑2268982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑