Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,287,796	C→T	L196L (CTG→CTA)	ccmF ←	cytochrome c‑type biogenesis heme lyase CcmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,287,796	0	C	T	100.0%	107.9 / NA	28	L196L (CTG→CTA)	ccmF	cytochrome c‑type biogenesis heme lyase CcmF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

GTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAG  >  NZ_CP009273/2287684‑2287925                                                                                                                 |                                                                                                                                  gTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCaaa                                                                                                         <  2:207880/139‑1 (MQ=255)  ttCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAAc                                                                                                        >  2:327989/1‑139 (MQ=255)       aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCAc                                                                                                   >  1:385445/1‑139 (MQ=255)                 tGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAAcc                                                                                          >  1:31026/1‑138 (MQ=255)                 tGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAAcc                                                                                          >  2:361120/1‑138 (MQ=255)                        ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  2:511418/139‑1 (MQ=255)                        ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  1:361120/139‑1 (MQ=255)                        ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  1:407723/139‑1 (MQ=255)                        ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  2:385445/139‑1 (MQ=255)                        ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  1:446104/139‑1 (MQ=255)                           aGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCa                                                                               >  1:20181/1‑139 (MQ=255)                           aGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCa                                                                               >  1:315510/1‑139 (MQ=255)                                     cGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGCGAAACCCACGTACCCCCTATAAAg                                                                        >  2:377703/1‑136 (MQ=255)                                       aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  >  2:450305/1‑124 (MQ=255)                                       aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc                                                                                  <  1:450305/124‑1 (MQ=255)                                          gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCg                                                                <  1:88127/139‑1 (MQ=255)                                              ggAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATg                                                            <  1:416545/139‑1 (MQ=255)                                                    tCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                      <  2:219811/139‑1 (MQ=255)                                                             ccAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCg                                              <  1:176249/138‑1 (MQ=255)                                                              cAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGa                                            <  2:20181/139‑1 (MQ=255)                                                              cAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGa                                            <  2:315510/139‑1 (MQ=255)                                                                       cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa                                    >  2:105566/1‑138 (MQ=255)                                                                                    cgcgCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                           >  1:187960/1‑134 (MQ=255)                                                                                      cgcATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCACTAGCGGGtt                           >  2:172004/1‑132 (MQ=255)                                                                                        cATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcgc                   >  1:528866/1‑138 (MQ=255)                                                                                                   tCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCg       >  1:510312/1‑139 (MQ=255)                                                                                                     cAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCggg     <  2:31026/139‑1 (MQ=255)                                                                                                        aCGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAg  >  1:429997/1‑139 (MQ=255)                                                                                                                 |                                                                                                                                  GTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAG  >  NZ_CP009273/2287684‑2287925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑