| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,290,049 | A→C | V152G (GTG→GGG) | ccmB ← | heme exporter protein CcmB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,290,049 | 0 | A | C | 92.3% | 38.0 / ‑2.6 | 13 | V152G (GTG→GGG) | ccmB | heme exporter protein CcmB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (6/6); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01 | |||||||||||
CCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGA > NZ_CP009273/2289924‑2290175 | ccAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCAc < 1:61200/139‑1 (MQ=255) cgcgTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGtt < 1:68758/139‑1 (MQ=255) cgTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTcc > 1:324052/1‑139 (MQ=255) cGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATcc < 1:411806/139‑1 (MQ=255) aGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCg < 1:561736/82‑1 (MQ=255) aGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCg > 2:561736/1‑82 (MQ=255) agcagcACACCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTa > 1:393995/1‑139 (MQ=255) cacaCCACCGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGt > 1:424693/1‑139 (MQ=255) caccGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAgcgc < 1:183042/85‑1 (MQ=255) caccGCGCTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAgcgc > 2:183042/1‑85 (MQ=255) cgcTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGa > 1:68479/1‑139 (MQ=255) cgcTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGa > 2:324099/1‑139 (MQ=255) cgcTTAAGTCCCCCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGa < 2:424693/139‑1 (MQ=255) | CCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGA > NZ_CP009273/2289924‑2290175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |