Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,306,184	T→C	V15V (GTA→GTG)	ompC ←	porin OmpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,306,184	0	T	C	100.0%	106.1 / NA	30	V15V (GTA→GTG)	ompC	porin OmpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

CATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGC  >  NZ_CP009273/2306058‑2306307                                                                                                                               |                                                                                                                            cATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCt                                                                                                                 <  2:568337/139‑1 (MQ=255)            ggTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCaggag                                                                                                      >  2:204592/1‑139 (MQ=255)             gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCaggagg                                                                                                     >  1:578108/1‑139 (MQ=255)             gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCagg                                                                                                        <  2:578108/136‑1 (MQ=255)             gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCagg                                                                                                        <  1:81128/136‑1 (MQ=255)                        cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                                                                                          <  2:110637/139‑1 (MQ=255)                              tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGAc                                                                                                  <  2:19616/125‑1 (MQ=255)                              tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGAc                                                                                                  >  1:19616/1‑125 (MQ=255)                                  gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                                                                                          >  1:142813/1‑129 (MQ=255)                                  gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                                                                                          <  2:142813/129‑1 (MQ=255)                                               tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCagg                                                                                                        <  2:239879/102‑1 (MQ=255)                                               tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCagg                                                                                                        >  1:239879/1‑102 (MQ=255)                                                                aCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat                                                                            >  2:255795/1‑113 (MQ=255)                                                                aCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat                                                                            <  1:255795/113‑1 (MQ=255)                                                                  cGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGc                                                >  2:267998/1‑139 (MQ=255)                                                                    tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTAt                                                    >  2:281630/1‑133 (MQ=255)                                                                    tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTAt                                                    <  1:281630/133‑1 (MQ=255)                                                                      cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttt                                            >  1:19506/1‑139 (MQ=255)                                                                              aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATg                                       <  2:221875/136‑1 (MQ=255)                                                                              aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATg                                       <  2:31209/136‑1 (MQ=255)                                                                                   ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGAc                                                                                                  <  1:556677/72‑1 (MQ=255)                                                                                   ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGAc                                                                                                  >  2:556677/1‑72 (MQ=255)                                                                                        gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACt                           >  1:63243/1‑138 (MQ=255)                                                                                             tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGAt                        <  1:326081/136‑1 (MQ=255)                                                                                               ttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAAc                    <  2:11468/138‑1 (MQ=255)                                                                                                                gTTTGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGc  <  1:204592/139‑1 (MQ=255)                                                                                                                 tttGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGc  >  2:278864/1‑138 (MQ=255)                                                                                                                 tttGCTGCGCCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGc  >  1:237115/1‑138 (MQ=255)                                                                                                                         gcCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATa                             >  1:297248/1‑103 (MQ=255)                                                                                                                         gcCTGCCACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATa                             <  2:297248/103‑1 (MQ=255)                                                                                                                               |                                                                                                                            CATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGC  >  NZ_CP009273/2306058‑2306307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑