Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	277,390	T→C	D102G (GAC→GGC)	yagA ←	IS481‑like element ISEc19 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	277,390	0	T	C	100.0%	199.3 / NA	58	D102G (GAC→GGC)	yagA	IS481‑like element ISEc19 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/26);  total (32/26)

CGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCG  >  NZ_CP009273/277280‑277511                                                                                                               |                                                                                                                          cgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGGAGCGCCCGGCAGCAGGCCATGGCCGGCCCTCAGGTTATGGAACGGGCTGGAAGCGGGCCTGGGGGGGCCCTGGCCCTCGGGCCAGCGCGTAACATTGCggg                                                                                                >  2:238020/1‑138 (MQ=255)      tCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTccc                                                                                           >  1:500384/1‑138 (MQ=255)              gCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCa                                                                                  >  1:88016/1‑139 (MQ=255)               cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCa                                                                                         >  1:514704/1‑131 (MQ=255)               cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCa                                                                                         <  2:514704/131‑1 (MQ=255)                 cGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                               <  2:107904/139‑1 (MQ=255)                 cGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                               <  1:404994/139‑1 (MQ=255)                      cGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTc                                                                          <  2:309815/139‑1 (MQ=255)                      cGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTc                                                                          <  2:347454/139‑1 (MQ=255)                        ggAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGGTATGGACGGGGCTGAAGGCGGGGATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGGTCCCCCGCGGTCATGACGGTCAt                                                                        >  2:234495/1‑139 (MQ=255)                           aTGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                                     <  1:550627/139‑1 (MQ=255)                                cGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                                                <  1:118951/139‑1 (MQ=255)                                cGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                                                <  1:352467/139‑1 (MQ=255)                                   tGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACg                                                              >  1:136686/1‑138 (MQ=255)                                          cccGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                                     <  1:314418/124‑1 (MQ=255)                                          cccGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                                     >  2:314418/1‑124 (MQ=255)                                             ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGcc                                                    >  2:562525/1‑138 (MQ=255)                                             ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGcc                                                    <  1:562525/138‑1 (MQ=255)                                               cagcagGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTTCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                                                 <  2:49470/139‑1 (MQ=255)                                                     gCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATgtcg                                           >  1:239488/1‑139 (MQ=255)                                                      ccATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATgtcgt                                          <  2:239047/139‑1 (MQ=255)                                                         tGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATgtcgt                                          >  1:265655/1‑136 (MQ=255)                                                               gCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGc                                 >  1:22975/1‑139 (MQ=255)                                                               gCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCATAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGc                                 >  2:382627/1‑139 (MQ=255)                                                                ccATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCg                                >  1:402981/1‑139 (MQ=255)                                                                  aTCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGt                              <  2:11818/139‑1 (MQ=255)                                                                           tGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCTAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAAt                     >  2:247373/1‑139 (MQ=255)                                                                           tGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAAt                     >  2:128845/1‑139 (MQ=255)                                                                                 gTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCGGTGATGTCGTCAGATGAGCGGTTCGGGGa                       <  2:267931/131‑1 (MQ=255)                                                                                 gTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGa                       >  1:267931/1‑131 (MQ=255)                                                                                 gTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  1:180855/1‑139 (MQ=255)                                                                                 gTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  1:122265/1‑139 (MQ=255)                                                                                 gTGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  1:139074/1‑139 (MQ=255)                                                                                  tGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  2:513293/1‑138 (MQ=255)                                                                                  tGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  1:479140/1‑138 (MQ=255)                                                                                  tGCTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCg               >  1:299789/1‑138 (MQ=255)                                                                                    cTGAAGGCGGGCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGa             <  1:234495/138‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                               <  1:181302/64‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                               >  2:181302/1‑64 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                                                >  2:69989/1‑79 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                                                <  1:69989/79‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                                  <  2:151607/109‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                                  >  1:151607/1‑109 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    <  2:217448/139‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    >  1:100983/1‑139 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    <  1:20805/139‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    >  1:226968/1‑139 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    >  1:463311/1‑139 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    <  1:576925/139‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    <  2:139074/139‑1 (MQ=255)                                                                                            gggCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcggg    <  2:22975/139‑1 (MQ=255)                                                                                              gCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCAt                                                                        >  1:68567/1‑69 (MQ=255)                                                                                              gCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCAt                                                                        <  2:68567/69‑1 (MQ=255)                                                                                              gCATGGTGTGCCCCTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcgggcg  >  2:66031/1‑139 (MQ=255)                                                                                                        ccccTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                                                 <  1:47840/82‑1 (MQ=255)                                                                                                        ccccTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                                                 >  2:47840/1‑82 (MQ=255)                                                                                                        ccccTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcgggcg  <  2:43734/129‑1 (MQ=255)                                                                                                        ccccTGGCCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCgcgggcg  >  1:43734/1‑129 (MQ=255)                                                                                                               |                                                                                                                          CGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCG  >  NZ_CP009273/277280‑277511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑