Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,345,762	A→G	F31S (TTC→TCC)	glpT ←	glycerol‑3‑phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,345,762	0	A	G	100.0%	128.6 / NA	36	F31S (TTC→TCC)	glpT	glycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/19);  total (17/19)

GATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCC  >  NZ_CP009273/2345638‑2345896                                                                                                                             |                                                                                                                                       gaTCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGAGCAAAGTTCTTACGAACCAAATAGTAAGCAGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAgcg                                                                                                                           >  2:563418/1‑138 (MQ=255)       cGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATcga                                                                                                                    >  2:215063/1‑139 (MQ=255)         aaaGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATcgacg                                                                                                                  <  1:485941/139‑1 (MQ=255)                        aaTCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGgatcg                                                                                                     <  1:563418/137‑1 (MQ=255)                            aCCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGAtctc                                                                                                <  1:72678/138‑1 (MQ=255)                              cgcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgc                                                                                             >  1:331697/1‑139 (MQ=255)                              cgcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgc                                                                                             >  1:383892/1‑139 (MQ=255)                                cgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCgg                                                                                           <  2:410423/139‑1 (MQ=255)                                                        tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcg                                                                   <  2:383892/139‑1 (MQ=255)                                                          aGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                                                  <  2:147565/138‑1 (MQ=255)                                                           ggCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTg                                                                >  1:42978/1‑139 (MQ=255)                                                                aGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgcg                                                                                            <  1:160385/106‑1 (MQ=255)                                                                aGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgcg                                                                                            >  2:160385/1‑106 (MQ=255)                                                                aGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGttt                                                            >  1:155726/1‑138 (MQ=255)                                                                aGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGttt                                                            >  2:456105/1‑138 (MQ=255)                                                                  cAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcg                                                                                              >  1:250289/1‑102 (MQ=255)                                                                  cAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcg                                                                                              <  2:250289/102‑1 (MQ=255)                                                                      cgcAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATa                                                     >  1:166651/1‑139 (MQ=255)                                                                      cgcAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATa                                                     >  1:186299/1‑139 (MQ=255)                                                                        cAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                                                   <  2:42978/139‑1 (MQ=255)                                                                                    gAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAg                                       <  1:507936/139‑1 (MQ=255)                                                                                     aaCCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGc                                      >  2:52842/1‑139 (MQ=255)                                                                                          aaTAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCg                                 <  2:155726/139‑1 (MQ=255)                                                                                          aaTAGTAAGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCg                                 <  1:463914/139‑1 (MQ=255)                                                                                                 aGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg                          <  2:528689/139‑1 (MQ=255)                                                                                                 aGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg                          <  1:456105/139‑1 (MQ=255)                                                                                                 aGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg                          <  2:318852/139‑1 (MQ=255)                                                                                                 aGCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg                          >  2:172173/1‑139 (MQ=255)                                                                                                  gCCGCATAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGt                         >  2:118735/1‑139 (MQ=255)                                                                                                       aTAGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCt                    >  2:183377/1‑139 (MQ=255)                                                                                                         aGCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGTTCTCCGCGGCAGGTAAGCGCGCTTTGTGCGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCtta                  >  2:208892/1‑139 (MQ=255)                                                                                                          gCCAAAGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCttat                 <  2:549032/139‑1 (MQ=255)                                                                                                              aaGAATATCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCttatttat             <  1:398638/139‑1 (MQ=255)                                                                                                                     tCCCCAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTaa       >  1:266750/1‑138 (MQ=255)                                                                                                                        ccAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGc   <  1:172173/139‑1 (MQ=255)                                                                                                                         cAGGGAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGcc  <  2:186299/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                       GATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCC  >  NZ_CP009273/2345638‑2345896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑