Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,352,068	A→C	V86G (GTC→GGC)	yfaU ←	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,352,068	0	A	C	100.0%	106.4 / NA	30	V86G (GTC→GGC)	yfaU	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/16);  total (14/16)

ACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGT  >  NZ_CP009273/2351950‑2352188                                                                                                                       |                                                                                                                         acTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTa                                                                                                      <  2:562063/139‑1 (MQ=255) acTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTa                                                                                                      <  1:131708/139‑1 (MQ=255) acTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTa                                                                                                      >  1:517595/1‑139 (MQ=255)     gCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGc                                                                                                  >  1:225169/1‑139 (MQ=255)     gCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGc                                                                                                  >  2:535204/1‑139 (MQ=255)     gCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTAGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGc                                                                                                  >  2:51153/1‑139 (MQ=255)                acGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACcgga                                                                                       <  2:538714/139‑1 (MQ=255)                acGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACcgga                                                                                       <  2:439716/139‑1 (MQ=255)                         gTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACg                                                                              <  2:319720/139‑1 (MQ=255)                            gggAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                                                            <  2:225169/138‑1 (MQ=255)                            gggAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                                                            <  2:353242/138‑1 (MQ=255)                                ggaTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtgg                                                                        >  2:377466/1‑138 (MQ=255)                                ggaTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtgg                                                                        <  1:377466/138‑1 (MQ=255)                                    aGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                                                            >  1:294111/1‑130 (MQ=255)                                    aGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                                                            <  2:294111/130‑1 (MQ=255)                                      cgcgTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCa                                                                  >  1:252082/1‑138 (MQ=255)                                         gTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAgg                                                              <  2:252082/139‑1 (MQ=255)                                           ggCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgc                                                            >  1:164188/1‑139 (MQ=255)                                           ggCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgc                                                            >  2:357027/1‑139 (MQ=255)                                                          aCGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGc                                             <  1:51153/139‑1 (MQ=255)                                                                   ttCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                                     <  2:164188/138‑1 (MQ=255)                                                                   ttCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                                     >  2:42126/1‑138 (MQ=255)                                                                   ttCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                                     <  1:25296/138‑1 (MQ=255)                                                                                                     tGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGt  >  1:565643/1‑139 (MQ=255)                                                                                                     tGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTCACTGCCTTCCACcgga                                                                                       >  2:197459/1‑54 (MQ=38)                                                                                                     tGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTCACTGCCTTCCACcgga                                                                                       <  1:197459/54‑1 (MQ=38)                                                                                                      gcgcgcCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                          <  2:245987/114‑1 (MQ=255)                                                                                                      gcgcgcCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                          >  1:245987/1‑114 (MQ=255)                                                                                                        gcgcCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCCCCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                                                        <  2:428095/82‑1 (MQ=255)                                                                                                        gcgcCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                                                        >  1:428095/1‑82 (MQ=255)                                                                                                                       |                                                                                                                         ACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGT  >  NZ_CP009273/2351950‑2352188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑