Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,357,677	A→G	G26G (GGT→GGC)	yfaZ ←	YfaZ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,357,677	0	A	G	100.0%	91.5 / NA	27	G26G (GGT→GGC)	yfaZ	YfaZ family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

AACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCC  >  NZ_CP009273/2357552‑2357812                                                                                                                              |                                                                                                                                        aaCCCCAGCCCCACGCCAGCGACATCCCCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAACCCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTca                                                                                                                            <  1:508349/139‑1 (MQ=255)      cacccccACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcattgca                                                                                                                       <  2:342417/136‑1 (MQ=255)         ccccACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcattgcatt                                                                                                                     >  1:476960/1‑138 (MQ=255)           ccACGCCAGCGACATCCCCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTACCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGAc                                                                                                                  <  2:159168/139‑1 (MQ=255)                 cAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATg                                                                                                             >  1:176592/1‑138 (MQ=255)                  aGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGAcc                                                                                                                 <  2:548700/133‑1 (MQ=255)                  aGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGAcc                                                                                                                 >  1:548700/1‑133 (MQ=255)                             cgtcgtcgtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 >  2:565440/1‑106 (MQ=255)                             cgtcgtcgtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 <  1:565440/106‑1 (MQ=255)                                               tCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCtac                                                                              >  2:360754/1‑137 (MQ=255)                                                         gCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtt                                                                    >  1:49162/1‑139 (MQ=255)                                                                          tcgtTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCtt                                                   >  1:148555/1‑139 (MQ=255)                                                                                               cacCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCaaa                              <  1:43723/139‑1 (MQ=255)                                                                                               cacCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCaa                               >  1:308343/1‑138 (MQ=255)                                                                                                               ttCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCgg              >  2:516120/1‑139 (MQ=255)                                                                                                                    tACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTg         >  2:493687/1‑139 (MQ=255)                                                                                                                     aCCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTg         >  2:53879/1‑138 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  2:324855/139‑1 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  1:516120/139‑1 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  2:476960/139‑1 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  1:172179/139‑1 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  1:493687/139‑1 (MQ=255)                                                                                                                      cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATGCCCAAAGCTGCGGGATTGTa       <  2:308343/139‑1 (MQ=255)                                                                                                                         gCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTg    >  1:402050/1‑139 (MQ=255)                                                                                                                          ccTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGc   >  1:311830/1‑139 (MQ=255)                                                                                                                          ccTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGc   >  2:465078/1‑139 (MQ=255)                                                                                                                           cTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGcc  <  1:53879/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                        AACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCC  >  NZ_CP009273/2357552‑2357812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑