| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,370,881 | A→G | V64A (GTC→GCC) | menH ← | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,370,881 | 0 | A | G | 100.0% | 50.0 / NA | 15 | V64A (GTC→GCC) | menH | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/7); total (8/7) | |||||||||||
ACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATC > NZ_CP009273/2370760‑2370996 | acaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATc > 2:25351/1‑138 (MQ=255) aaGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAaccacc < 2:314320/139‑1 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 1:189570/139‑1 (MQ=255) cccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAAc < 2:341010/139‑1 (MQ=255) aGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGt > 1:1223/1‑138 (MQ=255) cagccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa > 1:365866/1‑117 (MQ=255) cagccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa < 2:365866/117‑1 (MQ=255) gTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGcc > 1:486613/1‑138 (MQ=255) aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGaa > 1:182350/1‑79 (MQ=255) aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGaa < 2:182350/79‑1 (MQ=255) aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCAct > 2:539408/1‑139 (MQ=255) tgttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTg < 2:841/139‑1 (MQ=255) aGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc > 1:430631/1‑139 (MQ=255) tACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt > 1:91878/1‑72 (MQ=255) tACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 2:91878/72‑1 (MQ=255) | ACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATC > NZ_CP009273/2370760‑2370996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |