Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,438,026	T→C	K69E (AAA→GAA)	epmC ←	elongation factor P hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,438,026	0	T	C	100.0%	83.6 / NA	25	K69E (AAA→GAA)	epmC	elongation factor P hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

TATCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTTCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCTTTAATCAGAC  >  NZ_CP009273/2437890‑2438157                                                                                                                                         |                                                                                                                                    tatCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTccc                                                                                                                                   <  1:56679/139‑1 (MQ=255)   tCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  <  1:385399/138‑1 (MQ=255)   tCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  <  2:20702/138‑1 (MQ=255)   tCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  <  2:288737/138‑1 (MQ=255)                  gAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGc                                                                                                                  <  2:103881/139‑1 (MQ=255)                        ccAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAAtc                                                                                                            >  2:166270/1‑139 (MQ=255)                           gTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCa                                                                                                         >  1:428246/1‑139 (MQ=255)                                              acttcaACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  <  2:449557/95‑1 (MQ=255)                                              acttcaACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  >  1:449557/1‑95 (MQ=255)                                              acttcaACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTccc                                                                                                                                   >  1:344057/1‑94 (MQ=255)                                              acttcaACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTccc                                                                                                                                   <  2:344057/94‑1 (MQ=255)                                                  caACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGc                                                                                   >  2:429811/1‑138 (MQ=255)                                                   aaCATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCa                                                                                 <  1:383454/139‑1 (MQ=255)                                                        cTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAg                                                                             >  1:450314/1‑138 (MQ=255)                                                        cTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAg                                                                             >  2:531315/1‑138 (MQ=255)                                                          tCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCa                                                                           >  1:19811/1‑138 (MQ=255)                                                                  gCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGa                                                                    <  2:439957/137‑1 (MQ=255)                                                                  gCTTTGCGTATGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCACGCTACCCCGGCAATACACCAGTGCGAGATCTCATGAATTGCACTGGCATAAAAGCCATGGACAAAGACGAtt                                                                  <  2:509546/139‑1 (MQ=255)                                                                                      gtccgtccGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGc                                              <  2:174844/139‑1 (MQ=255)                                                                                         cgtccgGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCAt                                            >  2:538922/1‑138 (MQ=255)                                                                                              gggCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATcagcag                                      <  1:166270/139‑1 (MQ=255)                                                                                               ggCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATcagcag                                      >  1:519030/1‑138 (MQ=255)                                                                                                    tACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  >  2:413766/1‑41 (MQ=255)                                                                                                    tACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTcccc                                                                                                                                  <  1:413766/41‑1 (MQ=255)                                                                                                         gTAGCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAaga                                 >  1:488102/1‑133 (MQ=255)                                                                                                            gCCGAAATCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGt                        <  1:538922/139‑1 (MQ=255)                                                                                                                   tCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTtcgtcgt                 <  2:428246/139‑1 (MQ=255)                                                                                                                   tCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTtcgtcgt                 <  2:43829/139‑1 (MQ=255)                                                                                                                   tCAACCAGCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTtcgtcgt                 <  2:488102/139‑1 (MQ=255)                                                                                                                          gCTCACGGCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAATGCCATGAGCAACGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCtt           >  1:255173/1‑138 (MQ=255)                                                                                                                                ggCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGa                                                                    >  1:477582/1‑75 (MQ=255)                                                                                                                                ggCGCGCTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGa                                                                    <  2:477582/75‑1 (MQ=255)                                                                                                                                  cgcgcTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCTTTAATCAGAc  <  1:531315/139‑1 (MQ=255)                                                                                                                                  cgcgcTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCTTTAATCAGAc  <  1:507552/139‑1 (MQ=255)                                                                                                                                  cgcgcTTCCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCTTTAATCAGAc  <  1:156743/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TATCCCGCCGCCACACAGAACAACCAGTCGAGCGCCTGCGGCTTCACTTCAACATCTTCAAACTGGCTTTGCGTTTGGGCATCGCGTCCGTCCGGGCAATACCAGTAGCCGAAATCAACCAGCTCACGGCGCGCTTTCCCGGCAATACACCAGTGCGAAATCTCATGAATTGCGCTGGCATAAAAGCCATGAGCAAAGACGATTCGGTTATACGGTACTTCCGCATCAGCAGGAAGATAGATCGGTTCGTCGTCGCCTTTAATCAGAC  >  NZ_CP009273/2437890‑2438157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑