Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,453,160	T→G	K263N (AAA→AAC)	fadI ←	acetyl‑CoA C‑acyltransferase FadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,453,160	0	T	G	100.0%	67.4 / NA	20	K263N (AAA→AAC)	fadI	acetyl‑CoA C‑acyltransferase FadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/14);  total (6/14)

GTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCT  >  NZ_CP009273/2453037‑2453275                                                                                                                            |                                                                                                                    gTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGcc                                                                                                      <  1:118927/139‑1 (MQ=255)  tAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCg                                                                                                     >  1:549208/1‑139 (MQ=255)  tAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCg                                                                                                     >  2:215104/1‑139 (MQ=255)        cgcAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGgcg                                                                                                 <  2:512808/137‑1 (MQ=255)        cgcAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGgcg                                                                                                 >  1:235749/1‑137 (MQ=255)                ccccAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCg                                                                                       <  2:278068/139‑1 (MQ=255)                       ggCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCgg                                                                                <  1:215104/139‑1 (MQ=255)                         cACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCa                                                                              <  2:140865/139‑1 (MQ=255)                                          ccGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACcgc                                                              <  1:40884/137‑1 (MQ=255)                                            gCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGa                                                            <  2:13332/138‑1 (MQ=255)                                                 ggATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttg                                                      <  1:496223/139‑1 (MQ=255)                                                                          gCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgt                                    >  2:92787/1‑132 (MQ=255)                                                                          gCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgt                                    <  1:92787/132‑1 (MQ=255)                                                                              cgcCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                         <  2:330633/139‑1 (MQ=255)                                                                               gcCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagga                        <  2:437238/139‑1 (MQ=255)                                                                                              cgTACTGTTTGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGtc         <  1:174634/139‑1 (MQ=255)                                                                                                     tttGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                         <  2:139476/116‑1 (MQ=255)                                                                                                     tttGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                         >  1:139476/1‑116 (MQ=255)                                                                                                     tttGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCAcc   <  1:188742/138‑1 (MQ=255)                                                                                                     tttGCCGCCGTTACCGTTCCGTGGTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACct  >  2:398810/1‑139 (MQ=255)                                                                                                                            |                                                                                                                    GTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCT  >  NZ_CP009273/2453037‑2453275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑