| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,470,023 | A→G | intergenic (+34/+150) | torI → / ← dsdC | response regulator inhibitor TorI/DNA‑binding transcriptional regulator DsdC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,470,023 | 0 | A | G | 96.8% | 99.0 / ‑3.4 | 31 | intergenic (+34/+150) | torI/dsdC | response regulator inhibitor TorI/DNA‑binding transcriptional regulator DsdC |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (16/14); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01 | |||||||||||
AGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAA > NZ_CP009273/2469887‑2470140 | aGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAgca < 2:247286/139‑1 (MQ=255) gCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAgcac > 2:466891/1‑138 (MQ=255) gCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAgcac > 2:374182/1‑138 (MQ=255) aCCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCAtt < 1:216925/139‑1 (MQ=255) ccAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCcaat < 1:482342/139‑1 (MQ=255) ccAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCcaat < 1:444846/139‑1 (MQ=255) aaGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCctg > 2:151702/1‑139 (MQ=255) gggCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAg > 2:154480/1‑138 (MQ=255) cGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGa < 2:451382/139‑1 (MQ=255) aGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTg < 1:151702/139‑1 (MQ=255) atatCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCAGCTTCAAGTAGATGTCTGCAGGGGa < 1:374182/138‑1 (MQ=255) cGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCAtt < 2:314169/92‑1 (MQ=255) cGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCAtt > 1:314169/1‑92 (MQ=255) gACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGacac > 1:124529/1‑134 (MQ=255) gACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGacac < 2:124529/134‑1 (MQ=255) gACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATcc > 2:157758/1‑139 (MQ=255) aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCt > 2:69013/1‑139 (MQ=255) aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCt > 1:444713/1‑139 (MQ=255) aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCt > 2:372457/1‑139 (MQ=255) aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCt > 1:450240/1‑139 (MQ=255) tgtgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTgtgt > 1:562537/1‑139 (MQ=255) tgtgAATTCAAAAATAAGCTCTTAAGCCGCGCAAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTgtgt > 1:505874/1‑139 (MQ=255) tgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGttt < 2:570536/139‑1 (MQ=255) tCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATccc > 2:352315/1‑139 (MQ=255) aTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAAc < 1:18639/139‑1 (MQ=255) aTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAAc < 2:444713/139‑1 (MQ=255) cgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGta > 2:346623/1‑138 (MQ=255) cgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGta > 2:387737/1‑138 (MQ=255) ccAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTg < 2:216162/87‑1 (MQ=255) ccAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTg > 1:216162/1‑87 (MQ=255) tAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg < 1:154480/139‑1 (MQ=255) cGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGacac > 1:558675/1‑81 (MQ=255) cGGGTAAAATATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGacac < 2:558675/81‑1 (MQ=255) aaTATTTCTCACATCTAAAAAGCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGaa < 1:352315/139‑1 (MQ=255) | AGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAA > NZ_CP009273/2469887‑2470140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |