Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,487,351	A→G	D177D (GAT→GAC)	yfdX ←	YfdX family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,487,351	0	A	G	100.0%	90.0 / NA	26	D177D (GAT→GAC)	yfdX	YfdX family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

TACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCATTTTTTCATTGGCAATT  >  NZ_CP009273/2487221‑2487480                                                                                                                                   |                                                                                                                                  tACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCa                                                                                                                             <  2:238883/137‑1 (MQ=255)   ccTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTAGTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTg                                                                                                                         <  2:578055/139‑1 (MQ=255)       aaTATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTg                                                                                                                         <  2:395323/135‑1 (MQ=255)       aaTATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTg                                                                                                                         >  1:395323/1‑135 (MQ=255)                ccTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTc                                                                                                              <  1:12012/137‑1 (MQ=255)                   gTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGtt                                                                                                          >  1:50184/1‑138 (MQ=255)                          ttAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTgg                                                                                                   >  1:28323/1‑138 (MQ=255)                             gTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCAt                                                                                                <  1:174057/138‑1 (MQ=255)                                  caAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGt                                                                                                               <  1:126999/118‑1 (MQ=255)                                  caAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGt                                                                                                               >  2:126999/1‑118 (MQ=255)                                        gTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGtt                                                                                    <  1:451343/139‑1 (MQ=255)                                        gTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGtt                                                                                    >  1:252920/1‑139 (MQ=255)                                                   tCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTg                                                                                                                         >  1:75530/1‑91 (MQ=255)                                                   tCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTg                                                                                                                         <  2:75530/91‑1 (MQ=255)                                                                 aTCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAgg                                                           <  2:28323/139‑1 (MQ=255)                                                                  tCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGc                                                          >  1:150061/1‑139 (MQ=255)                                                                       cGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAg                                                      <  2:50184/138‑1 (MQ=255)                                                                       cGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGc                                                     >  2:195193/1‑139 (MQ=255)                                                                                     ccAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGcc                                       <  2:347120/139‑1 (MQ=255)                                                                                             gCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTa                                <  2:128317/138‑1 (MQ=255)                                                                                                              tttATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCAt                                                                              <  1:482102/75‑1 (MQ=255)                                                                                                              tttATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCAt                                                                              >  2:482102/1‑75 (MQ=255)                                                                                                                tATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCAtttttt             >  1:378149/1‑138 (MQ=255)                                                                                                                tATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCAtttttt             >  2:253569/1‑138 (MQ=255)                                                                                                                tATCAAGCAGTTTCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCAtttttt             >  2:374141/1‑138 (MQ=255)                                                                                                                          tttCTGCGCGTCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCATTTTTTCATTGGCAAtt  >  1:467582/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                  TACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGGCGAAGCTCTTCCATTGCGCCCTTTTTATCCCCTTTCGCCATTTTTTCATTGGCAATT  >  NZ_CP009273/2487221‑2487480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑