Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	281,176	A→T	Q24L (CAG→CTG)	yagG →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	281,176	0	A	T	100.0%	373.4 / NA	91	Q24L (CAG→CTG)	yagG	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (67/24);  total (67/24)

CTCATTACACTCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGG  >  NZ_CP009273/281045‑281304                                                                                                                                    |                                                                                                                                   cTCATTACACTCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTCCGAGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACg                                                                                                                             <  1:96880/139‑1 (MQ=255) cTCATTACACTCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCAc                                                                                                                              >  2:170761/1‑138 (MQ=255)     ttACACTCCATAAACACGATGTGTTAATTCGGCACACTAAATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGt                                                                                                                         <  2:135445/139‑1 (MQ=255)      tACACTCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGtt                                                                                                                        >  1:526497/1‑139 (MQ=255)           tCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   >  1:113242/1‑139 (MQ=255)           tCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   >  1:480695/1‑139 (MQ=255)           tCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   >  1:198372/1‑139 (MQ=255)           tCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   >  2:454470/1‑139 (MQ=255)                  acacGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTa                                                                                                            >  1:581461/1‑139 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCa                                                                                                                               <  2:392195/115‑1 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCa                                                                                                                               >  1:392195/1‑115 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTc                                                                                                      >  1:574466/1‑138 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTc                                                                                                      >  2:241062/1‑138 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTc                                                                                                      >  2:120133/1‑138 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTc                                                                                                      >  1:281676/1‑138 (MQ=255)                       aTGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTc                                                                                                      >  1:293089/1‑138 (MQ=255)                               aTTCGGCACACTACATAAGGGTGTAATTCTGATGACTCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGa                                                                                              <  1:537324/138‑1 (MQ=255)                               aTTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGa                                                                                              <  1:101657/138‑1 (MQ=255)                               aTTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGa                                                                                              <  1:241062/138‑1 (MQ=255)                                  cGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTc                                                                                          >  2:294733/1‑139 (MQ=255)                                  cGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTc                                                                                          >  1:411603/1‑139 (MQ=255)                                  cGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTc                                                                                          >  2:531181/1‑139 (MQ=255)                                          tACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCt                                                                                  >  1:499576/1‑139 (MQ=255)                                               aaGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTcgg                                                                             >  1:321771/1‑139 (MQ=255)                                                  ggTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTcggcgg                                                                          >  1:555175/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCACTTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:536162/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   >  2:190884/1‑98 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCctgc                                                                                                                   <  1:190884/98‑1 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGa                                                                                              >  1:38294/1‑117 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGa                                                                                              <  2:38294/117‑1 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  1:265647/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:191537/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:241897/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:251346/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:293044/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  2:572952/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCgggg                                                                        >  1:466703/1‑139 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:543705/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:169202/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:433486/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:202657/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:204346/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  1:358676/1‑138 (MQ=255)                                                    tgtAATTCTGATGACGCAATTAACCATGAAAGACAAAAGTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         <  2:433486/138‑1 (MQ=255)                                                    tgtAATTCTGATGAAGCAATTAACCATGAAAGACAAAATTGGCTACGAGCTGGGAGACACCGCCAGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCggg                                                                         >  2:39802/1‑138 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGcc                                                                                                              <  2:32733/101‑1 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGcc                                                                                                              >  1:32733/1‑101 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGag                                                                      >  1:155519/1‑138 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  2:71715/1‑139 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  2:506002/1‑139 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  2:220790/1‑139 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  2:534385/1‑139 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  1:307437/1‑139 (MQ=255)                                                      tAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGAt                                                                      >  1:435216/1‑139 (MQ=255)                                                         ttCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTAt                                                                   >  1:128905/1‑139 (MQ=255)                                                           cTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATgg                                                                 >  1:132064/1‑139 (MQ=255)                                                              atgaCGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATggg                                                                <  2:526497/137‑1 (MQ=255)                                                              atgaCGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCa                                                              >  1:178130/1‑139 (MQ=255)                                                               tgaCGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCAc                                                             <  1:429058/139‑1 (MQ=255)                                                                 aCGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACg                                                            <  2:293089/138‑1 (MQ=255)                                                                   gCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTg                                                         >  1:176909/1‑139 (MQ=255)                                                                   gCAATTAACCATGAAAGACAAAATTAGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTg                                                         >  1:97882/1‑139 (MQ=255)                                                                            cATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGtc                                                >  2:177231/1‑139 (MQ=255)                                                                               gAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTcc                                             >  1:382953/1‑139 (MQ=255)                                                                               gAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTcc                                             >  2:560083/1‑139 (MQ=255)                                                                                aaaGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTa                                                                    <  1:30495/115‑1 (MQ=255)                                                                                aaaGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTa                                                                    >  2:30495/1‑115 (MQ=255)                                                                                 aaGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTG‑TTTTGGTCTCCcgc                                          <  1:220790/139‑1 (MQ=255)                                                                                    aCAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGt                                        >  2:52191/1‑139 (MQ=255)                                                                                      aaaaTTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGc                                      >  1:243821/1‑139 (MQ=255)                                                                                             gCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACg                                <  2:555175/138‑1 (MQ=255)                                                                                              cTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGcc                              >  1:181419/1‑139 (MQ=255)                                                                                                  gggCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACg                                                                                                                             <  2:293945/42‑1 (MQ=255)                                                                                                  gggCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACg                                                                                                                             >  1:293945/1‑42 (MQ=255)                                                                                                   ggCTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGGTTTTGGGCACGCTGTTTTTGGTCTCCCGCGTGGTCGACGCCGTCAc                         >  2:419257/1‑139 (MQ=255)                                                                                                     cTGGGAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCg                       <  1:531181/139‑1 (MQ=255)                                                                                                        ggAGACTCCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGAc                     <  1:295284/138‑1 (MQ=255)                                                                                                        ggAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGAc                     <  1:534385/138‑1 (MQ=255)                                                                                                        ggAGACACCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGAc                     <  2:435216/138‑1 (MQ=255)                                                                                                              acCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGa              >  1:125734/1‑139 (MQ=255)                                                                                                              acCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGa              >  2:25834/1‑139 (MQ=255)                                                                                                              acCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGa              >  1:410/1‑139 (MQ=255)                                                                                                              acCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGa              >  1:535698/1‑139 (MQ=255)                                                                                                              acCGCCTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGa              >  1:513175/1‑139 (MQ=255)                                                                                                                  ccTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGCCGCCGTCACCGACCCGCTGATggg          >  2:480710/1‑139 (MQ=255)                                                                                                                  ccTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATggg          >  1:446963/1‑139 (MQ=255)                                                                                                                   cTGCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGGCCCGCTGATgggg         >  2:137329/1‑139 (MQ=255)                                                                                                                     gCGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGgct       >  2:133914/1‑139 (MQ=255)                                                                                                                      cGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGgc        <  2:466703/137‑1 (MQ=255)                                                                                                                      cGGCTTCGTCTGGCTGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGgc        <  2:281676/137‑1 (MQ=255)                                                                                                                             gTCTGGCTGGCCACGATGTTCCTGCTGGCCTATTTTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTgg  <  1:9360/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   CTCATTACACTCCATTAACACGATGTTGTAATTCGGCACACTACATAAGGGTGTAATTCTGATGACGCAATTAACCATGAAAGACAAAATTGGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTA‑‑TTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGG  >  NZ_CP009273/281045‑281304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑