Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,499,179	C→T	T311T (ACG→ACA)	ypdF ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,499,179	0	C	T	100.0%	114.6 / NA	29	T311T (ACG→ACA)	ypdF	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

CACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAC  >  NZ_CP009273/2499047‑2499307                                                                                                                                     |                                                                                                                                 cacTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCgtg                                                                                                                            <  2:58939/139‑1 (MQ=255) cacTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCgtg                                                                                                                            >  2:200548/1‑139 (MQ=255)      ttttCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTc                                                                                                                       >  2:360827/1‑139 (MQ=255)                ggCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGa                                                                                                              >  2:428385/1‑138 (MQ=255)                            ttCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                 >  2:529797/1‑139 (MQ=255)                            ttCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                 >  2:511225/1‑139 (MQ=255)                                 cgcCTTGCGGGATAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGACTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCAt                                                                                             >  2:474674/1‑138 (MQ=255)                                      tGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACtt                                                                                       >  2:213080/1‑139 (MQ=255)                                                ccAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGAt                                                                             <  1:168330/139‑1 (MQ=255)                                                    cacaACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                 <  1:368697/115‑1 (MQ=255)                                                    cacaACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                 >  2:368697/1‑115 (MQ=255)                                                         caTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAcc                                                                    <  1:114820/139‑1 (MQ=255)                                                         caTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAcc                                                                    <  1:200548/139‑1 (MQ=255)                                                                       aCGCCCCCTTGCCCTGGCAAATAAATCCCCGTCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCg                                                       >  1:307128/1‑138 (MQ=255)                                                                                     tGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATaa                                        >  2:326508/1‑139 (MQ=255)                                                                                       gCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAAcc                                      >  2:397680/1‑139 (MQ=255)                                                                                           ataaatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCt                                  <  1:360827/139‑1 (MQ=255)                                                                                              aatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTc                                <  1:386978/138‑1 (MQ=255)                                                                                                 ccccGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTg                                    >  1:53638/1‑131 (MQ=255)                                                                                                 ccccGGCTCCACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTg                                    <  2:53638/131‑1 (MQ=255)                                                                                                          cACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAAcc                                      >  2:17847/1‑120 (MQ=255)                                                                                                          cACGGTCAGTAACATGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAAcc                                      <  1:17847/120‑1 (MQ=255)                                                                                                                        tGCCTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGt      >  1:453393/1‑138 (MQ=255)                                                                                                                           cTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAc  >  2:84594/1‑139 (MQ=255)                                                                                                                           cTGGCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAc  >  2:124326/1‑139 (MQ=255)                                                                                                                             ggCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAg                                                 >  2:526933/1‑90 (MQ=255)                                                                                                                             ggCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAg                                                 <  1:526933/90‑1 (MQ=255)                                                                                                                             ggCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCgg            >  2:430343/1‑127 (MQ=255)                                                                                                                             ggCTGTAGTGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCgg            <  1:430343/127‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 CACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAC  >  NZ_CP009273/2499047‑2499307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑