Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,521,647	C→T	G630D (GGC→GAC)	ligA ←	NAD‑dependent DNA ligase LigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,521,647	0	C	T	100.0%	114.8 / NA	29	G630D (GGC→GAC)	ligA	NAD‑dependent DNA ligase LigA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/19);  total (10/19)

GCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCG  >  NZ_CP009273/2521523‑2521782                                                                                                                             |                                                                                                                                        gCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTcgcg                                                                                                                           <  2:65984/139‑1 (MQ=255)        aGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGtt                                                                                                                    >  1:11024/1‑139 (MQ=255)                cagcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGt                                                                                                            >  2:269752/1‑139 (MQ=255)                       tCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCt                                                                                                      <  1:388524/138‑1 (MQ=255)                                tcgATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGtcat                                                                                            <  1:352480/139‑1 (MQ=255)                                           aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:127846/138‑1 (MQ=255)                                           aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:184026/138‑1 (MQ=255)                                           aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:254136/138‑1 (MQ=255)                                           aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:269752/138‑1 (MQ=255)                                                      ccTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGc                                                                       <  1:373874/138‑1 (MQ=255)                                                      ccTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGc                                                                       <  2:61108/138‑1 (MQ=255)                                                       cTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTg                                                                     >  2:67647/1‑139 (MQ=255)                                                         gcgcCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:377331/124‑1 (MQ=255)                                                         gcgcCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  >  2:377331/1‑124 (MQ=255)                                                              ttCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGt                                                                >  1:65981/1‑137 (MQ=255)                                                                   cAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGCCCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGcacc                                                         <  2:241340/139‑1 (MQ=255)                                                                   cAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGcacc                                                         <  1:64673/139‑1 (MQ=255)                                                                   cAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTGCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCTCGCGACATCTGGCTTAAGCTGCCCGTAAGcacc                                                         <  1:176543/139‑1 (MQ=255)                                                                               tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTAc                                               >  1:512433/1‑137 (MQ=255)                                                                               tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTAc                                               >  2:321426/1‑137 (MQ=255)                                                                                gCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAg                                            >  1:438883/1‑139 (MQ=255)                                                                                        aCCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACggg                                    <  2:11024/139‑1 (MQ=255)                                                                                         cccGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGc                                   <  2:46497/139‑1 (MQ=255)                                                                                                    cAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATctct                        >  2:569174/1‑139 (MQ=255)                                                                                                       aTCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTcc                     >  2:569858/1‑139 (MQ=255)                                                                                                           gTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGt                 <  1:569858/139‑1 (MQ=255)                                                                                                                     acacGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGAt       <  2:306742/139‑1 (MQ=255)                                                                                                                     acacGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGAt       <  1:67647/139‑1 (MQ=255)                                                                                                                          cTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGgcg  <  2:532198/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                        GCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCG  >  NZ_CP009273/2521523‑2521782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑