Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,525,250	T→A	F143L (TTT→TTA)	cysZ →	sulfate transporter CysZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,525,250	0	T	A	100.0%	106.8 / NA	27	F143L (TTT→TTA)	cysZ	sulfate transporter CysZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/15);  total (12/15)

TGCCGCTCCGTTTAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTTGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATT  >  NZ_CP009273/2525118‑2525371                                                                                                                                     |                                                                                                                          tGCCGCTCCGTTTAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTgg                                                                                                                     >  2:70980/1‑139 (MQ=255)      cTCCGTTTAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCAAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGc                                                                                                                         <  1:399950/130‑1 (MQ=255)      cTCCGTTTAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCAAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGc                                                                                                                         >  2:399950/1‑130 (MQ=255)           tttAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCcgc                                                                                                           >  1:193706/1‑139 (MQ=255)               aCGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCgcgcgc                                                                                                       <  2:193706/139‑1 (MQ=255)                 ggTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCaa                                                                                                     <  1:70980/139‑1 (MQ=255)                   tttATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAAtt                                                                                                   >  2:10871/1‑139 (MQ=255)                      aTTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGt                                                                                                 <  1:10871/138‑1 (MQ=255)                          gCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTAc                                                                                            >  1:138695/1‑139 (MQ=255)                             gAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTaa                                                                                         >  2:283512/1‑139 (MQ=255)                                      gAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTAc                                                                                 <  1:391734/138‑1 (MQ=255)                                       aaGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACtt                                                                               <  1:449870/139‑1 (MQ=255)                                            cGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATcc                                                                          <  1:337297/139‑1 (MQ=255)                                             gATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATccc                                                                         <  1:93078/139‑1 (MQ=255)                                             gATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATccc                                                                         <  2:557105/139‑1 (MQ=255)                                                         cTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCa                                                             <  2:498891/139‑1 (MQ=255)                                                            cacCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCcgcg                                                                                                          <  1:85997/91‑1 (MQ=255)                                                            cacCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCcgcg                                                                                                          >  2:85997/1‑91 (MQ=255)                                                                 ccAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTAt                                                                                                                  >  2:463706/1‑78 (MQ=255)                                                                 ccAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTAt                                                                                                                  <  1:463706/78‑1 (MQ=255)                                                                         cGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTa                                             >  2:380640/1‑139 (MQ=255)                                                                                                   tgCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGAt                   >  1:514921/1‑139 (MQ=255)                                                                                                   tgCCGCGAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGAt                   >  1:483970/1‑139 (MQ=255)                                                                                                      cgcgAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGtt                <  2:514921/139‑1 (MQ=255)                                                                                                      cgcgAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGt                 >  2:558794/1‑138 (MQ=255)                                                                                                        cgAATCATGAAACGCGAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAg              <  1:93720/139‑1 (MQ=255)                                                                                                                    cgcgAATGGCAAAAATTAGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTAtt  <  1:558794/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                          TGCCGCTCCGTTTAACGGTTTATTGGCTGAACAACTGGAAGCACGATTGACTGGCGCTACACCGCCAGATACCGGGATTTTCGGTATCATGAAAGATGTGCCGCGAATCATGAAACGCGAATGGCAAAAATTTGCCTGGTATCTGCCGCGCGCAATTGTATTACTAATTCTTTACTTCATCCCTGGTATTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATT  >  NZ_CP009273/2525118‑2525371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑