Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,638,870	T→C	A605A (GCA→GCG)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,638,870	0	T	C	100.0%	58.7 / NA	19	A605A (GCA→GCG)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/13);  total (6/13)

CTTGCGGGCAATCAGCGGCAACACGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTAT  >  NZ_CP009273/2638743‑2639003                                                                                                                                |                                                                                                                                      cTTGCGGGCAATCAGCGGCAACACGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGGTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAggcagcg                                                                                                                            >  1:246801/1‑139 (MQ=255)        gCAATCAGCGGCAACACGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAg                                                                                                                     <  1:370842/139‑1 (MQ=255)                     acacGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCtt                                                                                                        <  1:35455/139‑1 (MQ=255)                     acacGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCtt                                                                                                        <  2:5815/139‑1 (MQ=255)                      cacGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTc                                                                                                       >  1:69732/1‑139 (MQ=255)                          tttGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGc                                                                                                   <  1:73584/139‑1 (MQ=255)                                          agccagATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCg                                                                                   <  1:515996/139‑1 (MQ=255)                                                    ggAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCACGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGAt                                                                         <  2:121514/139‑1 (MQ=255)                                                      aaaCGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATaa                                                                       >  1:97451/1‑139 (MQ=255)                                                          gAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATCGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGcc                                                                   <  2:97451/139‑1 (MQ=255)                                                          gAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGcc                                                                   <  2:182445/139‑1 (MQ=255)                                                                          ttCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTc                                                   >  2:412568/1‑139 (MQ=255)                                                                                     gCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTc                                        >  1:55402/1‑139 (MQ=255)                                                                                           aTAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCgg                                  <  2:365251/139‑1 (MQ=255)                                                                                                     cggcTGACTCCCGTCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCa                        <  1:412568/139‑1 (MQ=255)                                                                                                                  tCCAGCAGCCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTa           <  2:15916/139‑1 (MQ=255)                                                                                                                       cagcCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGcgcg                                            <  1:78171/101‑1 (MQ=255)                                                                                                                       cagcCAGGTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGcgcg                                            >  2:78171/1‑101 (MQ=255)                                                                                                                             ggTCGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGCCTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGATTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGAtattat  <  2:246801/137‑1 (MQ=255)                                                                                                                                |                                                                                                                                      CTTGCGGGCAATCAGCGGCAACACGTTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTAT  >  NZ_CP009273/2638743‑2639003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑