Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,658,192	A→C	Q148H (CAA→CAC)	yfhR →	alpha/beta hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,658,192	0	A	C	100.0%	63.0 / NA	19	Q148H (CAA→CAC)	yfhR	alpha/beta hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/12);  total (7/12)

GCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACAACGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAT  >  NZ_CP009273/2658057‑2658326                                                                                                                                        |                                                                                                                                       gCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGt                                                                                                                                     <  2:413781/139‑1 (MQ=255)  cGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTc                                                                                                                                    <  2:2836/139‑1 (MQ=255)                   tATGTTTGATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCa                                                                                                                    <  1:397386/138‑1 (MQ=255)                          gATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTg                                                                                                            >  1:452608/1‑139 (MQ=255)                                   gggTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGa                                                                                                   <  1:140596/139‑1 (MQ=255)                                    ggTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGaa                                                                                                  <  2:512387/139‑1 (MQ=255)                                                 aaaaGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGtt                                                                                     <  1:136383/139‑1 (MQ=255)                                                 aaaaGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGtt                                                                                     <  1:433860/139‑1 (MQ=255)                                                   aaGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGt                                                                                                                                <  2:122683/94‑1 (MQ=255)                                                   aaGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGt                                                                                                                                >  1:122683/1‑94 (MQ=255)                                                                        gATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCgg                                                                                                        <  1:542360/97‑1 (MQ=255)                                                                        gATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCgg                                                                                                        >  2:542360/1‑97 (MQ=255)                                                                        gATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGGATATTCTGGGTGGTATTGGTCGGGGGGGTCGTGGAgg                                                              >  2:289746/1‑139 (MQ=255)                                                                        gATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAgg                                                              <  2:452608/139‑1 (MQ=255)                                                                                              tGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGAc                                        >  1:356446/1‑139 (MQ=255)                                                                                               gCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGGGATCGTGAAGGCATACGTGCGGGGATCCTCGACt                                       >  1:500961/1‑139 (MQ=255)                                                                                                 cATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGGTGTTATTGGTCGGGGGGATCGTGAAGGCATACGTGCGGGGATCCTCGACTc                                      >  2:34215/1‑138 (MQ=255)                                                                                                       tgtgGTGCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACAttt                               <  2:339601/139‑1 (MQ=255)                                                                                                           gtgCGCCATCGCAGTGATGTAAACCCACACCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGcc                            <  1:289746/138‑1 (MQ=255)                                                                                                                                     acacCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTTGTCGGGGGGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTTCCTCTTATGCAACCATCGCCAACCAAAt  >  2:567418/1‑138 (MQ=255)                                                                                                                                     acacCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCgg                                                   <  2:217554/89‑1 (MQ=255)                                                                                                                                     acacCGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCgg                                                   >  1:217554/1‑89 (MQ=255)                                                                                                                                        |                                                                                                                                       GCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTTTGGTAAATCAAAAGGCACGCCGTCCCAGGCCGGATTGCTGGACGATACGCAAAGTGCCATCAATGTGGTGCGCCATCGCAGTGATGTAAACCCACAACGTCTGGTGCTGTTCGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAT  >  NZ_CP009273/2658057‑2658326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑