Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,666,371	T→A	D322E (GAT→GAA)	hcaD →	phenylpropionate dioxygenase ferredoxin reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,666,371	0	T	A	100.0%	63.2 / NA	17	D322E (GAT→GAA)	hcaD	phenylpropionate dioxygenase ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/7);  total (10/7)

TCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTCACGCT  >  NZ_CP009273/2666245‑2666502                                                                                                                               |                                                                                                                                    tCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGAt                                                                                                                         <  2:78535/139‑1 (MQ=255)                    cacCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGc                                                                                                       >  2:404578/1‑138 (MQ=255)                    cacCGCTGCGAAAGCTGGGAAAACGCCAACAACCAGGCGTAAATGGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTCCAGTTTATTGGc                                                                                                       <  2:237748/138‑1 (MQ=255)                       cGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGata                                                                                                   >  2:125526/1‑139 (MQ=255)                         cTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGa                                                                                                                          <  1:546613/114‑1 (MQ=255)                         cTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGa                                                                                                                          >  2:546613/1‑114 (MQ=255)                            cGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGt                                                                                              >  2:487275/1‑139 (MQ=255)                                          aCGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCt                                                                                <  2:27904/139‑1 (MQ=255)                                                                                ttAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGt                                          <  2:370226/139‑1 (MQ=255)                                                                                        accgctaccgctACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTg                                  >  2:534840/1‑139 (MQ=255)                                                                                          cgctaccgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTg                                                                                                         <  1:65303/66‑1 (MQ=255)                                                                                          cgctaccgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTg                                                                                                         >  2:65303/1‑66 (MQ=255)                                                                                          cgctaccgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGc                                 >  2:436596/1‑138 (MQ=255)                                                                                               ccgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACg                           >  2:569373/1‑139 (MQ=255)                                                                                                         cgccgccgTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTAt                 <  1:436596/139‑1 (MQ=255)                                                                                                                        ggAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTCACGCt  >  2:36266/1‑139 (MQ=255)                                                                                                                        ggAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTCACGCt  >  2:109470/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                    TCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTCACGCT  >  NZ_CP009273/2666245‑2666502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑