Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,699,422	A→C	R354R (CGT→CGG)	lepA ←	translation elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,699,422	0	A	C	100.0%	73.1 / NA	21	R354R (CGT→CGG)	lepA	translation elongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/8);  total (13/8)

TAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCG  >  NZ_CP009273/2699294‑2699548                                                                                                                                 |                                                                                                                               tAGATTTTATTTACCGCAGGCAGCTTTGATGGGCTTTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGga                                                                                                                      <  2:6977/139‑1 (MQ=255)    aTGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGgatga                                                                                                                   <  1:149088/139‑1 (MQ=255)       ttATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGAtct                                                                                                                >  2:568777/1‑139 (MQ=255)                 caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTgcag                                                                                                      <  1:560116/139‑1 (MQ=255)                        cTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGcc                                                                                                >  1:13/1‑138 (MQ=255)                                       gACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                                                                                 <  1:198134/138‑1 (MQ=255)                                            aGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAc                                                                            >  2:217453/1‑138 (MQ=255)                                                                                 tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 <  1:517248/128‑1 (MQ=255)                                                                                 tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 >  2:517248/1‑128 (MQ=255)                                                                                  aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 <  1:322776/127‑1 (MQ=255)                                                                                  aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 >  2:322776/1‑127 (MQ=255)                                                                                        ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAgg                               >  2:261523/1‑139 (MQ=255)                                                                                         gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAgg                               >  2:46900/1‑138 (MQ=255)                                                                                                    aTCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                   >  1:576895/1‑139 (MQ=255)                                                                                                    aTCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                   <  2:13/139‑1 (MQ=255)                                                                                                    aTCAGATCCAGATCGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                   >  2:555108/1‑139 (MQ=255)                                                                                                         atccagatcGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGt              >  1:36059/1‑139 (MQ=255)                                                                                                               atcGTATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTAcc         >  1:191127/1‑138 (MQ=255)                                                                                                                   tATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 <  1:375583/94‑1 (MQ=255)                                                                                                                   tATTCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                 >  2:375583/1‑94 (MQ=255)                                                                                                                     ttCACGTTCCAGCCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGcgcg  >  1:300506/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                               TAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCG  >  NZ_CP009273/2699294‑2699548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑