Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,745,770	A→G	E206G (GAG→GGG)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,745,770	0	A	G	100.0%	94.6 / NA	28	E206G (GAG→GGG)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/9);  total (19/9)

TTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACAC  >  NZ_CP009273/2745646‑2745896                                                                                                                             |                                                                                                                               ttGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcg                                                                                                                  <  2:369402/139‑1 (MQ=255) ttGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATc                                                                                                                   >  1:66804/1‑138 (MQ=255)    tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                               <  1:201610/139‑1 (MQ=255)    tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                               <  1:352327/139‑1 (MQ=255)    tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgac                                                                                                                >  1:105884/1‑138 (MQ=255)                  ccGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGt                                                                                                 <  1:243865/139‑1 (MQ=255)                         cTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCg                                                                                           >  1:220383/1‑138 (MQ=255)                            gcgcATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGaa                                                                                                             >  1:154639/1‑117 (MQ=255)                            gcgcATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGaa                                                                                                             <  2:154639/117‑1 (MQ=255)                                           ttAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGa                                                                        >  1:315191/1‑139 (MQ=255)                                           ttAAGTCAGGAACGCGCCACCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGa                                                                        >  1:421576/1‑139 (MQ=255)                                             aaGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGac                                                                       >  2:561935/1‑138 (MQ=255)                                                   ggAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGacca                                                                     <  1:6829/134‑1 (MQ=255)                                                   ggAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGacca                                                                     >  2:6829/1‑134 (MQ=255)                                                     aaCGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCAccagc                                                                >  2:542545/1‑137 (MQ=255)                                                              cccGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAGACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCAtt                                                     >  2:563564/1‑139 (MQ=255)                                                                                 aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGt                                  >  2:433998/1‑139 (MQ=255)                                                                                 aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGt                                  >  2:536230/1‑139 (MQ=255)                                                                                 aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGt                                  >  2:551144/1‑139 (MQ=255)                                                                                      aaTTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGCCATTAATGGCCGACGGTGAAGa                             >  2:433711/1‑139 (MQ=255)                                                                                       aTTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGAc                            >  2:202466/1‑139 (MQ=255)                                                                                                 cTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTg                   >  2:315760/1‑138 (MQ=255)                                                                                                 cTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTg                   >  1:117281/1‑138 (MQ=255)                                                                                                      cGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGt             <  1:121536/139‑1 (MQ=255)                                                                                                         aTTTAATCCTCAGCCCGGAGGTTTTTAACAAATCTACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCCCCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACTCAAACCTGCAAAGTCAg          <  1:555209/139‑1 (MQ=255)                                                                                                         aTTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAg          >  1:429843/1‑139 (MQ=255)                                                                                                         aTTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAg          >  2:444961/1‑139 (MQ=255)                                                                                                                 cGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTacac  <  1:202466/139‑1 (MQ=255)                                                                                                                             |                                                                                                                               TTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACAC  >  NZ_CP009273/2745646‑2745896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑