Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,772,143	A→G	S1315P (TCC→CCC)	ypjA ←	adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,772,143	0	A	G	100.0%	111.1 / NA	32	S1315P (TCC→CCC)	ypjA	adhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

TTAAAACGGTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACGG  >  NZ_CP009273/2772013‑2772270                                                                                                                                   |                                                                                                                                ttAAAACGGTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCa                                                                                                                         >  1:163449/1‑139 (MQ=255)              gCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAg                                                                                                            <  2:234862/139‑1 (MQ=255)                    cGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTgg                                                                                                       >  2:287012/1‑138 (MQ=255)                       cacCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAAt                                                                                                   >  1:338889/1‑139 (MQ=255)                                    gAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAat                                                                                       <  1:263907/138‑1 (MQ=255)                                                   ttCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTc                                                                        <  2:369460/138‑1 (MQ=255)                                                     cccAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGa                                                                      <  2:426027/138‑1 (MQ=255)                                                      ccAACTGGCATAGCCCCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATTGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGAt                                                                     <  2:320386/138‑1 (MQ=255)                                                        aaCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                  <  2:23263/139‑1 (MQ=255)                                                                  gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                  >  1:122223/1‑129 (MQ=255)                                                                  gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                  <  2:122223/129‑1 (MQ=255)                                                                     gccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCg                                                     >  1:58297/1‑139 (MQ=255)                                                                                     cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                  >  2:421992/1‑110 (MQ=255)                                                                                     cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                  <  1:421992/110‑1 (MQ=255)                                                                                     cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCa                                      >  2:16219/1‑138 (MQ=255)                                                                                     cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCa                                      >  2:271763/1‑138 (MQ=255)                                                                                               cATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCAcc                           <  2:58297/139‑1 (MQ=255)                                                                                                   tcctccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCa                       >  2:349990/1‑139 (MQ=255)                                                                                                     ctccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCAt                      <  1:271763/138‑1 (MQ=255)                                                                                                      tccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAat                                                                                       <  1:40748/72‑1 (MQ=255)                                                                                                      tccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAat                                                                                       >  2:40748/1‑72 (MQ=255)                                                                                                        cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTc                                                  <  2:308983/107‑1 (MQ=255)                                                                                                        cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTc                                                  >  1:308983/1‑107 (MQ=255)                                                                                                        cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggt                  >  2:529144/1‑139 (MQ=255)                                                                                                        cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggt                  >  2:364875/1‑139 (MQ=255)                                                                                                        cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATACCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggt                  >  2:173153/1‑139 (MQ=255)                                                                                                          cgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggtgg                <  1:97593/139‑1 (MQ=255)                                                                                                                      atatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGCGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTAc    >  2:96663/1‑139 (MQ=255)                                                                                                                         tgtgAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACgg  <  2:338889/138‑1 (MQ=255)                                                                                                                         tgtgAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACgg  <  1:16219/138‑1 (MQ=255)                                                                                                                             aaTGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGc                              <  2:196750/106‑1 (MQ=255)                                                                                                                             aaTGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGc                              >  1:196750/1‑106 (MQ=255)                                                                                                                                   |                                                                                                                                TTAAAACGGTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACGG  >  NZ_CP009273/2772013‑2772270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑