Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,829,895	A→G	V297A (GTC→GCC)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,829,895	0	A	G	100.0%	70.9 / NA	22	V297A (GTC→GCC)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGCGTCTGGTAAACCGT  >  NZ_CP009273/2829775‑2830012                                                                                                                         |                                                                                                                       gcCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAgggg                                                                                                      <  1:575444/139‑1 (MQ=255)        cGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGatag                                                                                                <  2:53613/138‑2 (MQ=255)          ttGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATatcat                                                                                             >  2:330471/1‑139 (MQ=255)                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGatat                                                                                                >  2:536483/1‑130 (MQ=255)                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGatat                                                                                                <  1:536483/130‑1 (MQ=255)                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATcaca                                                                                         <  2:411297/137‑1 (MQ=255)                               ccGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGa                                                                                                   <  2:60037/112‑1 (MQ=255)                               ccGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGa                                                                                                   >  1:60037/1‑112 (MQ=255)                                                           gCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTTATCCACCAGCACAATcggcgcgg                                            >  2:178340/1‑139 (MQ=255)                                                           gCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATcggcgcggc                                           >  1:74872/1‑139 (MQ=255)                                                                     cAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTa                                 <  2:489022/139‑1 (MQ=255)                                                                                aCAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAgg                                                                                >  2:238201/1‑82 (MQ=255)                                                                                aCAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAgg                                                                                <  1:238201/82‑1 (MQ=255)                                                                                    cAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGgcgcg                    >  2:133528/1‑137 (MQ=255)                                                                                    cAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGgcgcg                    >  1:502989/1‑137 (MQ=255)                                                                                    cAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGGAAGCAACTTgcgcg                    >  2:524951/1‑137 (MQ=255)                                                                                     aaaTGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAg                 >  1:247136/1‑139 (MQ=255)                                                                                       aTGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGCg               >  2:302676/1‑139 (MQ=255)                                                                                              aGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGCGTCTGGTa        <  2:502989/139‑1 (MQ=255)                                                                                                  ggTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGc                >  2:244099/1‑127 (MQ=255)                                                                                                  ggTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGc                <  1:244099/127‑1 (MQ=255)                                                                                                      cGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGCGTCTGGTAAACCGt  <  1:178340/138‑1 (MQ=255)                                                                                                                         |                                                                                                                       GCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTA‑TTTTTTATCCACCAGCACAATCGGCGCGGCGGGCGTGGTAAGCAACTGGCGCGCAGCGTCTGGTAAACCGT  >  NZ_CP009273/2829775‑2830012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑