Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,891,751	T→G	T343P (ACC→CCC)	ygcU ←	FAD‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,891,751	0	T	G	100.0%	100.7 / NA	29	T343P (ACC→CCC)	ygcU	FAD‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/10);  total (19/10)

CGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCC  >  NZ_CP009273/2891625‑2891880                                                                                                                               |                                                                                                                                  cGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTg                                                                                                                       >  1:475446/1‑139 (MQ=255)    ccAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGca                                                                                                                     >  2:308403/1‑138 (MQ=255)        cATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATACCGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTg                                                                                                                               <  2:56289/124‑1 (MQ=255)        cATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATACCGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTg                                                                                                                               >  1:56289/1‑124 (MQ=255)               aTGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAg                                                                                                         <  1:474551/139‑1 (MQ=255)                  tcgtcgGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCa                                                                                                      >  2:186871/1‑139 (MQ=255)                       ggCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACttt                                                                                                  <  2:62041/138‑1 (MQ=255)                       ggCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTa                                                                                                 >  1:380360/1‑139 (MQ=255)                               ggAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcc                                                                                         <  2:11996/139‑1 (MQ=255)                                 aaCTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcccc                                                                                       <  1:186647/139‑1 (MQ=255)                                             aTACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgttgt                                                                           >  2:545604/1‑139 (MQ=255)                                             aTACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgttgt                                                                           >  2:427094/1‑139 (MQ=255)                                              tACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgttgt                                                                           >  1:239800/1‑138 (MQ=255)                                                cGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTa                                                                         >  2:151455/1‑138 (MQ=255)                                                  gTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAAc                                                                      >  1:206577/1‑139 (MQ=255)                                                             tttCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                                           >  2:407134/1‑139 (MQ=255)                                                               tCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                                           >  1:44426/1‑137 (MQ=255)                                                               tCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                                           >  2:222398/1‑137 (MQ=255)                                                                         cGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTc                                               >  1:129846/1‑139 (MQ=255)                                                                          gTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTc                                               <  1:222398/138‑1 (MQ=255)                                                                                            gcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCggg                            >  2:239447/1‑139 (MQ=255)                                                                                            gcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCggg                            >  2:511205/1‑139 (MQ=255)                                                                                                       cGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAAcc                                                                     >  2:409325/1‑87 (MQ=255)                                                                                                       cGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAAcc                                                                     <  1:409325/87‑1 (MQ=255)                                                                                                          tggtAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGAt              <  1:368816/139‑1 (MQ=255)                                                                                                                aGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGc        <  1:427094/139‑1 (MQ=255)                                                                                                                 gCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCa       <  1:64157/139‑1 (MQ=255)                                                                                                                      tGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATccc  >  2:342562/1‑139 (MQ=255)                                                                                                                      tGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATccc  >  1:467110/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                  CGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCC  >  NZ_CP009273/2891625‑2891880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑