Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,931,836	A→G	D337G (GAT→GGT)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,931,836	0	A	G	100.0%	122.5 / NA	35	D337G (GAT→GGT)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

CAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCG  >  NZ_CP009273/2931704‑2931962                                                                                                                                     |                                                                                                                               caggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCg                                                                                                                           >  1:326414/1‑138 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATg                                                                                                                   >  2:469101/1‑138 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATg                                                                                                                   <  1:329960/138‑1 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATg                                                                                                                   <  2:444694/138‑1 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATg                                                                                                                   >  2:33734/1‑138 (MQ=255)              cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAgtg                                                                                                              <  2:476201/138‑1 (MQ=255)              cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAgtg                                                                                                              >  1:476201/1‑138 (MQ=255)                        cAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGt                                                                                                   >  1:547635/1‑139 (MQ=255)                          aTGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCg                                                                                                 <  2:408233/139‑1 (MQ=255)                             gctgccATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCgtgt                                                                                              >  1:121351/1‑139 (MQ=255)                                ggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCgtgt                                                                                              >  2:303944/1‑136 (MQ=255)                                 gcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAg                                                                                           >  1:523742/1‑138 (MQ=255)                                    tCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACg                                                                                       <  1:303539/139‑1 (MQ=255)                                    tCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACg                                                                                       <  1:303944/139‑1 (MQ=255)                                           tGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctggctg                                                                                >  2:581694/1‑139 (MQ=255)                                                gAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCa                                                                             <  1:33734/137‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                      <  1:581694/139‑1 (MQ=255)                                                       tGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGAc                                                                    >  1:122059/1‑139 (MQ=255)                                                               cTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATa                                                            >  1:529351/1‑139 (MQ=255)                                                               cTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATa                                                            >  1:524536/1‑139 (MQ=255)                                                                  ttCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATAcc                                                          <  2:326414/138‑1 (MQ=255)                                                                            cTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCa                                               >  1:508308/1‑139 (MQ=255)                                                                            cTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCa                                               >  2:577168/1‑139 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgcg                                    <  1:469101/137‑1 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgcg                                    <  2:529351/137‑1 (MQ=255)                                                                                          cAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCgcgg                                 <  2:566618/139‑1 (MQ=255)                                                                                                   attgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAg                        >  1:440256/1‑139 (MQ=255)                                                                                                                 tCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAgt                                                                                                               <  2:216560/38‑1 (MQ=255)                                                                                                                 tCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAgt                                                                                                               >  1:216560/1‑38 (MQ=255)                                                                                                                 tCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCaa          >  1:65599/1‑139 (MQ=255)                                                                                                                  cTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                      <  1:414846/78‑1 (MQ=255)                                                                                                                  cTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                      >  2:414846/1‑78 (MQ=255)                                                                                                                      tCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACa     <  2:421087/139‑1 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg  <  2:122059/139‑1 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg  <  1:269960/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                               CAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCG  >  NZ_CP009273/2931704‑2931962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑