Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,938,251	T→C	D117D (GAT→GAC)	csdE →	cysteine desulfurase sulfur acceptor subunit CsdE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,938,251	0	T	C	100.0%	74.5 / NA	22	D117D (GAT→GAC)	csdE	cysteine desulfurase sulfur acceptor subunit CsdE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

GCAAAATGCATTTCTTTGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATC  >  NZ_CP009273/2938124‑2938382                                                                                                                                |                                                                                                                                    gCAAAATGCATTTCTTTGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGAtt                                                                                                                          <  2:287692/139‑1 (MQ=255)         cATTTCTTTGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTgcg                                                                                                                   <  1:247775/138‑1 (MQ=255)         cATTTCTTTGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTgcg                                                                                                                   <  2:485121/138‑1 (MQ=255)                 tGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCCGGCACAGTCACCACTGGCATTTTTTGACGAGCTGGGATTACGTGCGCAGCTTAgc                                                                                                          >  1:264095/1‑139 (MQ=255)                 tGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTa                                                                                                            <  2:502216/137‑1 (MQ=255)                        aGCGAAGGGCGCATTGTGCGCGGCCTTCTGTCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCAACACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCAc                                                                                                   <  1:164782/139‑1 (MQ=255)                               ggCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCa                                                                                            <  2:199840/139‑1 (MQ=255)                                      tgtgCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAAGGGAAAACCGCCGCCGAGTTGCCGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTTCGCAGCTTAGCGCCTCACGCAGCCAGGGGtt                                                                                      >  1:428592/1‑138 (MQ=255)                                                  gctgGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCAAGGGGTTAAATGCGTTAAGc                                                                         >  2:295216/1‑139 (MQ=255)                                                   ctgGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGc                                                                         <  1:207124/138‑1 (MQ=255)                                                      gCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCAAGGGGTTAAATGCGTTAAGCGAg                                                                      <  1:295216/138‑1 (MQ=255)                                                                    tGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGc                                                        >  1:458286/1‑138 (MQ=255)                                                                    tGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGc                                                        >  2:363398/1‑138 (MQ=255)                                                                      ccGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGcgac                                                     >  1:25191/1‑139 (MQ=255)                                                                      ccGTTGAGGGG‑AAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGGTAAGCGAGGCGATTATCGCTGcgac                                                     >  1:40910/1‑138 (MQ=255)                                                                           gAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAg                                                 <  1:175668/138‑1 (MQ=255)                                                                                 aaaaCCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGttt                                          <  2:264095/139‑1 (MQ=255)                                                                                 aaaaCCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGttt                                          <  1:363398/139‑1 (MQ=255)                                                                                                                      cATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGa                      <  2:333520/122‑1 (MQ=255)                                                                                                                      cATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGa                      >  1:333520/1‑122 (MQ=255)                                                                                                                      cATTGTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct      >  2:453746/1‑138 (MQ=255)                                                                                                                          gTTTGACGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc  <  2:458286/138‑1 (MQ=255)                                                                                                                                |                                                                                                                                    GCAAAATGCATTTCTTTGGCGACAGCGAAGGGCGCATTGTGCGCGGCCTGCTGGCGGTGTTGTTGACTGCCGTTGAGGGGAAAACCGCCGCCGAGTTGCAGGCACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATC  >  NZ_CP009273/2938124‑2938382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑