Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,005,362	T→C	G181G (GGT→GGC)	arcC →	carbamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,005,362	0	T	C	100.0%	85.1 / NA	24	G181G (GGT→GGC)	arcC	carbamate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/17);  total (7/17)

AGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGTCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCGATATGCT  >  NZ_CP009273/3005235‑3005496                                                                                                                                |                                                                                                                                       agCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGAt                                                                                                                             <  1:390930/139‑1 (MQ=255)          cAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTgg                                                                                                                    <  1:493297/139‑1 (MQ=255)            aaaCAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCg                                                                                                                  <  1:13973/139‑1 (MQ=255)             aaCAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCgg                                                                                                                 <  1:273956/139‑1 (MQ=255)             aaCAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCgg                                                                                                                 <  1:35199/139‑1 (MQ=255)                                     aTGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGa                                                                                         <  1:366356/139‑1 (MQ=255)                                      tGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTg                                                                                          >  1:209964/1‑137 (MQ=255)                                            tCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGcaggccag                                                                                                                                     >  1:217597/1‑88 (MQ=255)                                            tCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGcaggccag                                                                                                                                     <  2:217597/88‑1 (MQ=255)                                                ggCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCGGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATc                                                                              <  1:421103/139‑1 (MQ=255)                                                     tCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                                                                          <  2:102879/138‑1 (MQ=255)                                                            gttgttGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGc                                                                    >  1:299158/1‑137 (MQ=255)                                                                     tCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                                                         >  2:23913/1‑139 (MQ=255)                                                                       gCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGAt                                                                                                                             <  2:345557/69‑1 (MQ=255)                                                                       gCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGAt                                                                                                                             >  1:345557/1‑69 (MQ=255)                                                                       gCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTCATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                                                         <  2:299158/137‑1 (MQ=255)                                                                                 gTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                                                         >  1:415557/1‑127 (MQ=255)                                                                                 gTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                                                         <  2:415557/127‑1 (MQ=255)                                                                                         cATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATa                                                   <  1:218202/125‑1 (MQ=255)                                                                                         cATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATa                                                   >  2:218202/1‑125 (MQ=255)                                                                                                        ggTTAAAGCTCTGGTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGaa                      <  1:30621/139‑1 (MQ=255)                                                                                                                     gTAGATGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCg         <  2:501416/139‑1 (MQ=255)                                                                                                                         aTGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCGatat     <  1:434298/139‑1 (MQ=255)                                                                                                                         aTGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCGatat     <  1:394856/139‑1 (MQ=255)                                                                                                                          tGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGAt                                                    >  2:373132/1‑91 (MQ=255)                                                                                                                          tGCAGGCCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGAt                                                    <  1:373132/91‑1 (MQ=255)                                                                                                                             aggccaggtGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCGATATGCt  >  1:248827/1‑138 (MQ=255)                                                                                                                                |                                                                                                                                       AGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGCCGTGTTGTTGCCTCGCCAAAACCGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGTCAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGGGCCAGCGCCCGTTTAGCAGAAATGATTGATGCCGATATGCT  >  NZ_CP009273/3005235‑3005496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑