| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,036,314 | A→G | F65S (TTC→TCC) | yqfA ← | hemolysin III family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,036,314 | 0 | A | G | 96.2% | 82.7 / ‑6.1 | 26 | F65S (TTC→TCC) | yqfA | hemolysin III family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (12/13); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCC > NZ_CP009273/3036183‑3036438 | aaTCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGGAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATAGCGTGATAGAGCGTCGAAGCGAGGGAcagcag > 1:497762/1‑139 (MQ=255) aaaaCGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATa < 2:218997/139‑1 (MQ=255) cGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTg > 1:50142/1‑139 (MQ=255) tCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAg > 2:579725/1‑133 (MQ=255) tCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAg < 1:579725/133‑1 (MQ=255) cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcg > 2:475169/1‑139 (MQ=255) cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCAtt > 1:101524/1‑134 (MQ=255) ttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGAt < 1:292797/68‑1 (MQ=255) ttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGAt > 2:292797/1‑68 (MQ=255) cAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCa < 1:30000/137‑1 (MQ=255) tttttGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAacac < 2:50142/139‑1 (MQ=255) cGTTGATGAGGAATGCGGTGATAGAGCGTGAAAGGGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACCCCAGCCCaa < 2:512231/139‑1 (MQ=255) gTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa > 2:97504/1‑102 (MQ=255) gTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa < 1:97504/102‑1 (MQ=255) gTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAAc > 2:551254/1‑139 (MQ=255) ttGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAAc > 2:497020/1‑138 (MQ=255) ttGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTACCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACg < 2:101524/139‑1 (MQ=255) gatgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGa < 1:184913/138‑1 (MQ=255) gatgaGGAATGGCGTGATAGAGCAACGAAGCGAGGGAGAGCGGGATAATACTGCCGCCATAGAGGCTGTAGCTGGGTATCGCTGGGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGa < 2:400455/138‑1 (MQ=255) aGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCa < 1:497020/139‑1 (MQ=255) tGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac > 1:210425/1‑139 (MQ=255) aTAGAGCGTCTAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAAccc > 2:186143/1‑138 (MQ=255) aTAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCa < 2:179167/139‑1 (MQ=255) gCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGcc < 1:73226/139‑1 (MQ=255) gAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCa < 2:154179/117‑1 (MQ=255) gAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCa > 1:154179/1‑117 (MQ=255) | AATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCC > NZ_CP009273/3036183‑3036438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |