Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,071,778	A→C	G147G (GGT→GGG)	cmtA ←	PTS mannitol transporter subunit IICB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,071,778	0	A	C	100.0%	106.3 / NA	30	G147G (GGT→GGG)	cmtA	PTS mannitol transporter subunit IICB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/14);  total (16/14)

CATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCC  >  NZ_CP009273/3071642‑3071906                                                                                                                                         |                                                                                                                                 cATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAccc                                                                                                                                >  2:265409/1‑139 (MQ=255)           gAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACa                                                                                                                       >  1:126184/1‑138 (MQ=255)                 cccTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGc                                                                                                                <  2:316349/137‑1 (MQ=255)                   cTTTCGCCGCTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTAACGAAAGTATTGGCAATTAACCCCGCCGGGCCGAGAACTTCAAACCCCAGCAGACAAAGGAGCAt                                                                                                              <  2:141247/139‑1 (MQ=255)                         ccGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                        <  1:40264/139‑1 (MQ=255)                         ccGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                        <  1:306616/139‑1 (MQ=255)                                       actgacAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgag                                                                                           >  2:389282/1‑138 (MQ=255)                                           acaacaATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGt                                                                                       >  1:581795/1‑138 (MQ=255)                                               caaTGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTAtt                                                                                   >  1:157162/1‑138 (MQ=255)                                                       gATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCAt                                                                          >  1:34893/1‑139 (MQ=255)                                                                      ccAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAgg                                                           >  1:358890/1‑139 (MQ=255)                                                                        aGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTg                                                                                  <  1:526370/114‑1 (MQ=255)                                                                        aGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTg                                                                                  >  2:526370/1‑114 (MQ=255)                                                                          cgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATg                                                       >  1:112363/1‑139 (MQ=255)                                                                              tCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGtt                                                   >  2:123209/1‑139 (MQ=255)                                                                                    cacTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCa                                             <  1:265409/139‑1 (MQ=255)                                                                                       tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTg                                          >  1:195060/1‑139 (MQ=255)                                                                                         tttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACa                                                                                                                       <  1:504204/60‑1 (MQ=255)                                                                                         tttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACa                                                                                                                       >  2:504204/1‑60 (MQ=255)                                                                                           tGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCt                                       >  1:385134/1‑138 (MQ=255)                                                                                                         gCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATAtttt                         <  1:49376/138‑1 (MQ=255)                                                                                                         gCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATAtttt                         >  2:49376/1‑138 (MQ=255)                                                                                                              tAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGAc                    >  1:396851/1‑138 (MQ=255)                                                                                                                     gccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCAc            >  1:114438/1‑139 (MQ=255)                                                                                                                      ccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGt                                                    >  2:167433/1‑98 (MQ=255)                                                                                                                      ccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGt                                                    <  1:167433/98‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg        <  2:385134/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg        <  2:143887/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg        <  2:581795/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTGCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg        <  2:386479/139‑1 (MQ=255)                                                                                                                               tAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGccc  <  2:569438/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCC  >  NZ_CP009273/3071642‑3071906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑