Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,148,355	A→G	S56P (TCC→CCC)	yqhC ←	DNA‑binding transcriptional regulator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,148,355	0	A	G	100.0%	106.3 / NA	30	S56P (TCC→CCC)	yqhC	DNA‑binding transcriptional regulator YqhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

GACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTAT  >  NZ_CP009273/3148220‑3148477                                                                                                                                        |                                                                                                                           gaCGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGgaaa                                                                                                                         <  2:69896/139‑1 (MQ=255) gaCGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGgaaa                                                                                                                         <  1:129426/139‑1 (MQ=255)            gCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTatga                                                                                                              >  1:446833/1‑139 (MQ=255)               aGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGc                                                                                                           <  1:130587/139‑1 (MQ=255)               aGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGc                                                                                                           <  1:177364/139‑1 (MQ=255)               aGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGc                                                                                                           <  2:302237/139‑1 (MQ=255)                    cACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGc                                                                                                      <  1:453968/139‑1 (MQ=255)                               tGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCAc                                                                                           <  2:69645/139‑1 (MQ=255)                               tGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCAc                                                                                           <  2:310986/139‑1 (MQ=255)                                 gCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCg                                                                                         >  2:481839/1‑139 (MQ=255)                                          tCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTg                                                                                >  1:551671/1‑139 (MQ=255)                                           cGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGc                                                                               >  2:292395/1‑139 (MQ=255)                                                      ggcaacggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGtct                                                                    <  1:96862/139‑1 (MQ=255)                                                                ccGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaa                                                          >  2:69587/1‑139 (MQ=255)                                                                  gTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaac                                                         >  2:242932/1‑138 (MQ=255)                                                                   tCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaaca                                                        >  2:122583/1‑138 (MQ=255)                                                                          cagGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGcaca                                                <  2:372064/139‑1 (MQ=255)                                                                           agGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACAt                                               <  1:481839/139‑1 (MQ=255)                                                                            gggaTTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATc                                              >  1:23270/4‑139 (MQ=255)                                                                                   aTTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACACCAAACGCACATCGGGCaa                                        <  2:132388/138‑1 (MQ=255)                                                                                   aTTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        <  2:23270/138‑1 (MQ=255)                                                                                   aTTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        <  2:446833/138‑1 (MQ=255)                                                                                    ttGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACaaa                                                      <  1:503623/123‑1 (MQ=255)                                                                                    ttGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACaaa                                                      >  2:503623/1‑123 (MQ=255)                                                                                    ttGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        >  1:485434/1‑137 (MQ=255)                                                                                             tAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGAc                              >  1:412586/1‑138 (MQ=255)                                                                                                 gAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACt                         <  1:122583/139‑1 (MQ=255)                                                                                                                  atatAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTc         <  1:69587/138‑1 (MQ=255)                                                                                                                      aaCCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGca                                                  <  2:226013/93‑1 (MQ=255)                                                                                                                      aaCCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGca                                                  >  1:226013/1‑93 (MQ=255)                                                                                                                        ccGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttat  >  1:116076/1‑139 (MQ=255)                                                                                                                        ccGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttat  <  2:40313/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           GACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTAT  >  NZ_CP009273/3148220‑3148477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑