Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,155,922	A→G	V36A (GTT→GCT)	ftsP ←	cell division protein FtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,155,922	0	A	G	100.0%	159.3 / NA	48	V36A (GTT→GCT)	ftsP	cell division protein FtsP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

GACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTA  >  NZ_CP009273/3155786‑3156052                                                                                                                                         |                                                                                                                                   gACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCg                                                                                                                                  >  2:316966/1‑139 (MQ=255)    gcgGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCggg                                                                                                                                >  2:357484/1‑138 (MQ=255)       gctAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGc                                                                                                                            >  1:492839/3‑139 (MQ=255)                       tAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCgg                                                                                                            <  2:579858/139‑1 (MQ=255)                               aTTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                                                                    <  2:106446/139‑1 (MQ=255)                                             ccGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      <  2:470722/139‑1 (MQ=255)                                             ccGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      <  1:268920/139‑1 (MQ=255)                                             ccGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      <  2:576230/139‑1 (MQ=255)                                                aCGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgc                                                                                   >  1:240363/1‑139 (MQ=255)                                                aCGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgc                                                                                   >  1:230272/1‑139 (MQ=255)                                                aCGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGGAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGGACAgc                                                                                   >  1:512312/1‑139 (MQ=255)                                                 cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcg                                                                                                       >  2:522810/1‑118 (MQ=255)                                                 cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcg                                                                                                       <  1:522810/118‑1 (MQ=255)                                                         tCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca                                                                          <  2:554187/139‑1 (MQ=255)                                                              ggCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTg                                                                     >  1:206195/1‑139 (MQ=255)                                                              ggCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTg                                                                     >  2:351543/1‑139 (MQ=255)                                                                  tAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGca                                                                            >  1:449149/1‑128 (MQ=255)                                                                  tAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGca                                                                            <  2:449149/128‑1 (MQ=255)                                                                    aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGcc                                                                                                 >  2:202496/1‑105 (MQ=255)                                                                    aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGcc                                                                                                 <  1:202496/105‑1 (MQ=255)                                                                                    gTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatgaa                                               <  2:68500/139‑1 (MQ=255)                                                                                       gTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACt                                             <  1:351543/138‑1 (MQ=255)                                                                                              cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGa                                     >  2:50235/1‑139 (MQ=255)                                                                                              cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGa                                     >  1:35221/1‑139 (MQ=255)                                                                                                tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                   >  1:192185/1‑139 (MQ=255)                                                                                                tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                   >  2:424045/1‑139 (MQ=255)                                                                                                  aaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                   >  1:330113/1‑137 (MQ=255)                                                                                                      gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa                             <  2:230272/139‑1 (MQ=255)                                                                                                      gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa                             <  1:202570/139‑1 (MQ=255)                                                                                                      gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGAAGCCTGAATGAACTGACGCCGACTGAGTGa                             <  2:209810/139‑1 (MQ=255)                                                                                                       cGGTTGCCGACGGCGAGATTCAAGTAGCGGCGGGGCGGGTAGCGGTTGCGGTTGCCCGGCTGCGGTGGGCTTCAGGGGGACAGCGGCTGGACAAGGTGCAGTCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa                             >  2:428922/1‑138 (MQ=255)                                                                                                       cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGAAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa                             >  1:507567/1‑138 (MQ=255)                                                                                                         gTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      >  2:457133/1‑79 (MQ=255)                                                                                                         gTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      <  1:457133/79‑1 (MQ=255)                                                                                                          ttGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      <  2:34331/78‑1 (MQ=255)                                                                                                          ttGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                                                                      >  1:34331/1‑78 (MQ=255)                                                                                                                aCGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt                   <  2:34995/139‑1 (MQ=255)                                                                                                                aCGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt                   <  1:50235/139‑1 (MQ=255)                                                                                                                aCGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt                   <  1:225407/139‑1 (MQ=255)                                                                                                                 cGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGGTGCTGTTGCCCGGCTGCGCCTGCCTTCAGGGGAACAGCGCCCGCACAAAGGGCAATCCCCGGTGCCCGGATGGAATGACGCCGACTGAGGTGCATATTTGCTc                  >  2:225407/1‑139 (MQ=255)                                                                                                                           cAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTaaaa         <  2:283571/138‑1 (MQ=255)                                                                                                                             aGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTGAAACTg      <  2:512312/139‑1 (MQ=255)                                                                                                                               tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc    <  2:192185/139‑1 (MQ=255)                                                                                                                               tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc    <  2:171263/139‑1 (MQ=255)                                                                                                                               tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc    <  2:330113/139‑1 (MQ=255)                                                                                                                               tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc    <  2:462657/139‑1 (MQ=255)                                                                                                                               tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc    <  2:209898/139‑1 (MQ=255)                                                                                                                                  cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTa  >  1:181945/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                   GACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTA  >  NZ_CP009273/3155786‑3156052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑