Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	441,393	A→C	F244V (TTC→GTC)	yajR ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	441,393	0	A	C	100.0%	107.4 / NA	31	F244V (TTC→GTC)	yajR	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/15);  total (16/15)

ACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCAT  >  NZ_CP009273/441257‑441525                                                                                                                                         |                                                                                                                                     aCGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAccc                                                                                                                                    <  1:338182/139‑1 (MQ=255)             aCGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCa                                                                                                                        <  2:86165/139‑1 (MQ=255)                       cAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCggg                                                                                                              <  1:347856/139‑1 (MQ=255)                                gCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGcaa                                                                                                      >  1:563195/1‑138 (MQ=255)                                gCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGcaa                                                                                                      >  1:321622/1‑138 (MQ=255)                                      ttttGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCa                                                                                                                        >  1:171738/1‑114 (MQ=255)                                      ttttGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCa                                                                                                                        <  2:171738/114‑1 (MQ=255)                                           cgcTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACa                                                                                          >  1:218976/1‑139 (MQ=255)                                             cTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATc                                                                                        <  2:153981/139‑1 (MQ=255)                                                  cTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCaa                                                                                   <  1:311268/139‑1 (MQ=255)                                                     cAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCaaaa                                                                                 >  1:510247/1‑138 (MQ=255)                                                        cGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATg                                                                             >  2:55917/1‑139 (MQ=255)                                                        cGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATg                                                                             <  1:349026/139‑1 (MQ=255)                                                             aTAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGa                                                                         >  1:187017/1‑138 (MQ=255)                                                             aTAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGa                                                                         <  2:187017/138‑1 (MQ=255)                                                                  gAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACAt                                                                    >  1:155427/1‑138 (MQ=255)                                                                                       ggCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTcag                                               >  2:398115/1‑138 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATg                                                                             >  1:547270/1‑104 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATg                                                                             <  2:547270/104‑1 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGcc                                          >  1:564429/1‑139 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGcc                                          >  1:51983/1‑139 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGcc                                          >  2:556944/1‑139 (MQ=255)                                                                                           aTTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGcc                                          >  2:319667/1‑139 (MQ=255)                                                                                                    gTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTcc                                  <  2:321622/138‑1 (MQ=255)                                                                                                       gCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCa                              <  2:218976/139‑1 (MQ=255)                                                                                                       gCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCa                              <  2:51983/139‑1 (MQ=255)                                                                                                                      cAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGaaa                <  1:162337/138‑1 (MQ=255)                                                                                                                      cAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGaaa                <  1:398115/138‑1 (MQ=255)                                                                                                                      cAGTGTTCAGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAAc               <  2:155427/139‑1 (MQ=255)                                                                                                                              aGCCGCCGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCttt        >  1:255492/1‑138 (MQ=255)                                                                                                                               gccgccGGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCa      <  1:213939/139‑1 (MQ=255)                                                                                                                                   ccgGGACCCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCAt  >  2:368008/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     ACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCAT  >  NZ_CP009273/441257‑441525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑