Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,193,900	A→G	L142P (CTC→CCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,193,900	0	A	G	100.0%	81.8 / NA	24	L142P (CTC→CCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/11);  total (13/11)

CGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193771‑3194033                                                                                                                                  |                                                                                                                                      cGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGAttt                                                                                                                              >  1:285338/1‑139 (MQ=255) cGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGAttt                                                                                                                              >  2:465171/1‑139 (MQ=255)     aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAt                                                                                                                          <  2:71227/139‑1 (MQ=255)     aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAt                                                                                                                          <  2:102689/139‑1 (MQ=255)         ggTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTgg                                                                                                                      <  2:499359/139‑1 (MQ=255)         ggTTTGCGATACCAGTTGGTTCGCCAGTTGCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCGCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTgg                                                                                                                      <  2:492108/139‑1 (MQ=255)                                              gcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaa                                                                                  >  2:98219/1‑138 (MQ=255)                                               cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaa                                                                                 >  1:254625/1‑138 (MQ=255)                                                 cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  1:477467/139‑1 (MQ=255)                                                            gcTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAACTTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGaa                                                                    >  2:73469/1‑138 (MQ=255)                                                            gcTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAAc                                                                   <  1:31760/139‑1 (MQ=255)                                                                     ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTg                                                           >  1:4870/1‑138 (MQ=255)                                                                       ccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCa                                                         <  1:551495/138‑1 (MQ=255)                                                                          gttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGccac                                                                                                     <  1:556553/91‑1 (MQ=255)                                                                          gttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGccac                                                                                                     >  2:556553/1‑91 (MQ=255)                                                                               agCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAgg                                                >  2:49567/1‑139 (MQ=255)                                                                                     caGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCgg                                                    <  2:23573/129‑1 (MQ=255)                                                                                     caGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCgg                                                    >  1:23573/1‑129 (MQ=255)                                                                                         tAGGTTCAGCAAATTCACCCGCTTTCTCTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTA‑CATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATcggcg                                     <  1:268016/139‑1 (MQ=255)                                                                                           ggTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATcggcgg                                    >  1:331566/1‑139 (MQ=255)                                                                                              tCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGc                                  >  2:23007/1‑138 (MQ=255)                                                                                                                       cccccTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGAGGGCAATTCGCCGTTCGGGCCGAATTCCGt        >  1:449959/1‑139 (MQ=255)                                                                                                                         cccTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCg      >  2:469476/1‑139 (MQ=255)                                                                                                                              gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  <  2:4870/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      CGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193771‑3194033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑