Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,202,275 T→G V298G (GTG→GGG)  ttdT → L‑tartrate/succinate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,202,2750TG96.3% 89.1 / ‑2.8 27V298G (GTG→GGG) ttdTL‑tartrate/succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (11/15);  minor base C (0/1);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCT  >  NZ_CP009273/3202153‑3202409
                                                                                                                          |                                                                                                                                      
gTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGCGGATTTTcggc                                                                                                                        >  2:550417/1‑139 (MQ=255)
 tACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggc                                                                                                                        <  1:313610/138‑1 (MQ=255)
   cccGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       <  1:63375/137‑1 (MQ=255)
      gccgGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGAt                                                                                                                  >  1:359951/1‑139 (MQ=255)
           tACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTatat                                                                                                             >  1:485330/1‑139 (MQ=255)
                  gTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGcc                                                                                                      >  1:9501/1‑139 (MQ=255)
                  gTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGG‑ACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCcg                                                                                                     >  1:142649/1‑139 (MQ=255)
                       gCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa                                                                                                  <  2:359951/138‑1 (MQ=255)
                        cGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                                                                                <  1:538490/139‑1 (MQ=255)
                        cGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                                                                                <  1:521906/139‑1 (MQ=255)
                          aTCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGt                                                                                              <  2:359634/139‑1 (MQ=255)
                                  ccGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACa                                                                                      <  1:45878/139‑1 (MQ=255)
                                   cgcgCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGCGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAg                                                                                     <  1:515076/139‑1 (MQ=255)
                                          ggCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtggt                                                                               <  1:415513/138‑1 (MQ=255)
                                          ggCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtggt                                                                               >  2:415513/1‑138 (MQ=255)
                                               agaCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACt                                                                         <  2:88200/139‑1 (MQ=255)
                                                          cAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           <  1:391813/126‑1 (MQ=255)
                                                          cAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           >  2:391813/1‑126 (MQ=255)
                                                                             gTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGga                                           >  2:139614/1‑139 (MQ=255)
                                                                              ttCGCGTGGAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGAGTTTCGGCGGTGATTATATCGATGCCGCGATGTTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGgac                                          <  2:341823/139‑1 (MQ=255)
                                                                                  cgTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACa                                      >  1:403537/1‑139 (MQ=255)
                                                                                  cgTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACa                                      <  1:139614/139‑1 (MQ=255)
                                                                                         aaCGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAg                               <  2:403537/139‑1 (MQ=255)
                                                                                                 cTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATaaa                        <  2:485330/138‑1 (MQ=255)
                                                                                                          aTGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGa              <  2:142649/139‑1 (MQ=255)
                                                                                                                      cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTtcttct  >  1:29299/1‑139 (MQ=255)
                                                                                                                      cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTtcttct  >  1:192697/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                      
GTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCT  >  NZ_CP009273/3202153‑3202409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑