Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,210,830	A→C	intergenic (+69/+85)	nfeR → / ← aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,210,830	0	A	C	100.0%	108.4 / NA	31	intergenic (+69/+85)	nfeR/aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/14);  total (17/14)

GCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGT  >  NZ_CP009273/3210696‑3210958                                                                                                                                       |                                                                                                                                 gCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCg                                                                                                                              >  1:202971/1‑139 (MQ=255) gCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCg                                                                                                                              >  2:291414/1‑139 (MQ=255) gCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCg                                                                                                                              >  1:31546/1‑139 (MQ=255)   acaAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGc                                                                                                                             >  2:1044/1‑138 (MQ=255)     aaaTAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTa                                                                                                                           >  1:492212/1‑138 (MQ=255)       aTAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTaca                                                                                                                         >  1:142420/1‑138 (MQ=255)       aTAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTaca                                                                                                                         >  1:275522/1‑138 (MQ=255)        tAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTacaca                                                                                                                       <  1:38114/139‑1 (MQ=255)             aaGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  <  1:96895/139‑1 (MQ=255)             aaGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  <  1:428364/139‑1 (MQ=255)                 tCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAgg                                                                                                              >  1:236077/1‑139 (MQ=255)                     tGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCt                                                                                                           >  1:71023/1‑138 (MQ=255)                             tCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATg                                                                                                  >  2:563530/1‑139 (MQ=255)                                    cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  <  2:202646/116‑1 (MQ=255)                                    cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  <  2:253196/116‑1 (MQ=255)                                    cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  >  1:253196/1‑116 (MQ=255)                                    cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCg                                                                                                                  >  1:202646/1‑116 (MQ=255)                                    cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAg                                                                                            >  1:12136/1‑138 (MQ=255)                                      ccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcat                                                                                         <  1:238662/139‑1 (MQ=255)                                           tttGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcatcgcat                                                                                    >  1:377380/1‑139 (MQ=255)                                            ttGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcatcgcat                                                                                    <  2:492212/138‑1 (MQ=255)                                            ttGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcatcgcat                                                                                    >  1:214466/1‑138 (MQ=255)                                               atatTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAg                                                                                 <  1:289730/138‑1 (MQ=255)                                                                                  gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                             <  2:71023/139‑1 (MQ=255)                                                                                  gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                             <  2:214466/139‑1 (MQ=255)                                                                                  gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                             <  2:142420/139‑1 (MQ=255)                                                                                      cgcgGTGCAATTGCCGGATGCGACGCTTGCCGCGCCTTATCCGGCCTACCCCCGCTACACACCTCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAAt                                         <  1:257749/139‑1 (MQ=255)                                                                                        cgGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATg                                        >  1:455929/1‑138 (MQ=255)                                                                                        cgGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGc                                       <  2:12136/139‑1 (MQ=255)                                                                                                                            aTCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGgcgc     <  2:455929/137‑1 (MQ=255)                                                                                                                             tCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGgt  >  1:514830/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 GCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGT  >  NZ_CP009273/3210696‑3210958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑