Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,232,610	T→C	I224I (ATT→ATC)	alx →	TerC family membrane protein Alx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,232,610	0	T	C	100.0%	92.4 / NA	27	I224I (ATT→ATC)	alx	TerC family membrane protein Alx
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

ATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAA  >  NZ_CP009273/3232485‑3232736                                                                                                                              |                                                                                                                               aTGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCg                                                                                                                   >  2:426922/1‑139 (MQ=255)   gACCGACACCATCGACAACGAGCATTTCTTTGTGCGT‑AGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCg                                                                                                                >  2:51852/1‑139 (MQ=255)          acCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCa                                                                                                          <  2:412605/139‑1 (MQ=255)            cATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACt                                                                                                        <  1:525318/139‑1 (MQ=255)              tCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGa                                                                                                      <  2:285186/139‑1 (MQ=255)              tCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGa                                                                                                      <  1:385135/139‑1 (MQ=255)                              cTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCt                                                                                       <  1:340153/138‑1 (MQ=255)                              cTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCt                                                                                       <  2:553570/138‑1 (MQ=255)                                               taTTTCTTTACACCACACCGCTGATGCTGGTACTAATTCTTATTGAATTTAGCTACTTCATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTACTCACCTCAAACCTGTTTGc                                                                     <  1:396695/138‑1 (MQ=255)                                               gATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGc                                                                     <  1:300089/139‑1 (MQ=255)                                                    cTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATcc                                                                >  2:470686/1‑139 (MQ=255)                                                     tGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCt                                                               >  1:252441/1‑139 (MQ=255)                                                              cacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcg                                                      >  2:19533/1‑139 (MQ=255)                                                              cacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcg                                                      >  1:390594/1‑139 (MQ=255)                                                              cacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcg                                                      >  2:370455/1‑139 (MQ=255)                                                                 cGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCCTTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCTtgcgtgc                                                   >  2:377587/1‑139 (MQ=255)                                                                              aCTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATG‑ATTTCctgc                                     >  1:92114/1‑139 (MQ=255)                                                                                  aTTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGc                                                                     <  1:285911/104‑1 (MQ=255)                                                                                  aTTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGc                                                                     >  2:285911/1‑104 (MQ=255)                                                                                  aTTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTgg                                  >  1:491959/1‑139 (MQ=255)                                                                                       ggtggAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTAtt                                           >  2:193721/1‑125 (MQ=255)                                                                                       ggtggAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTAtt                                           <  1:193721/125‑1 (MQ=255)                                                                                                   cGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCctgctg                                   <  2:565173/121‑1 (MQ=255)                                                                                                   cGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCctgctg                                   >  1:565173/1‑121 (MQ=255)                                                                                                      cGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCCAAGCGTTtc              >  2:199459/1‑139 (MQ=255)                                                                                                          aTTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGa          <  1:19533/139‑1 (MQ=255)                                                                                                                  cGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCaaa  <  2:491959/139‑1 (MQ=255)                                                                                                                              |                                                                                                                               ATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAA  >  NZ_CP009273/3232485‑3232736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑