Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,326,882	A→G	intergenic (‑72/‑187)	rplU ← / → ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,326,882	0	A	G	100.0%	141.3 / NA	40	intergenic (‑72/‑187)	rplU/ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/19);  total (21/19)

GGCGAACGGTCTGACCTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCC  >  NZ_CP009273/3326747‑3327014                                                                                                                                        |                                                                                                                                     ggCGAACGGTCTGACCTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCGATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGata                                                                                                                                   >  1:91515/1‑139 (MQ=255)               ccTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  2:520277/139‑1 (MQ=255)               ccTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  2:280959/139‑1 (MQ=255)               ccTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  1:536234/139‑1 (MQ=255)                   cGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGcc                                                                                                                 >  2:296407/1‑139 (MQ=255)                        aCTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  2:496605/130‑1 (MQ=255)                        aCTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     >  1:496605/1‑130 (MQ=255)                        aCTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTg                                                                                                             <  1:286824/138‑1 (MQ=255)                         cTCGGTGTTGGTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACCCCTTTTCAATGACTCAGCGTGCGCTATAAATATTCACAATAGAGCGCGGATATTACGCATACCGCACGCCGTTGAc                                                                                                           <  2:141640/139‑1 (MQ=255)                                        caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCaa                                                                                            >  2:96107/1‑139 (MQ=255)                                          ccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt                                                                                                    <  2:265300/129‑1 (MQ=255)                                          ccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt                                                                                                    >  1:265300/1‑129 (MQ=255)                                             cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                                                                           <  2:459849/119‑1 (MQ=255)                                             cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                                                                           >  1:459849/1‑119 (MQ=255)                                                  gAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaag                                                                                   <  1:12183/138‑1 (MQ=255)                                                       ccGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  2:138119/99‑1 (MQ=255)                                                       ccGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     >  1:138119/1‑99 (MQ=255)                                                        cgcgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaa                                                                             >  1:563131/1‑138 (MQ=255)                                                              cATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAcaca                                                                       <  2:563131/138‑1 (MQ=255)                                                                 ataAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACtt                                                                   >  1:392895/1‑139 (MQ=255)                                                                       cTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACgg                                                             <  2:182226/139‑1 (MQ=255)                                                                           gCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAAc                                                         >  2:133735/1‑139 (MQ=255)                                                                                            ttCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt                                          <  2:460164/137‑1 (MQ=255)                                                                                                   aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACgg                                                             <  2:559897/111‑1 (MQ=255)                                                                                                   aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACgg                                                             >  1:559897/1‑111 (MQ=255)                                                                                                   aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                  >  1:311349/1‑138 (MQ=255)                                                                                                   aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                  >  2:61494/1‑138 (MQ=255)                                                                                                      cAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTAc                               <  2:311349/138‑1 (MQ=255)                                                                                                        gagTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAAt                            <  2:152768/139‑1 (MQ=255)                                                                                                             cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     >  2:251931/1‑45 (MQ=255)                                                                                                             cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                     <  1:251931/45‑1 (MQ=255)                                                                                                             cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAccc                       >  2:303756/1‑139 (MQ=255)                                                                                                             cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc                        <  1:444779/138‑1 (MQ=255)                                                                                                               cTATAAATATTCACAATAGGGAGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                     >  2:98026/1‑139 (MQ=255)                                                                                                                     atatTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTcc               >  1:76913/1‑139 (MQ=255)                                                                                                                       atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCt                      >  2:238330/1‑130 (MQ=255)                                                                                                                       atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCt                      <  1:238330/130‑1 (MQ=255)                                                                                                                          cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc           >  2:191711/1‑138 (MQ=255)                                                                                                                          cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc           >  1:562597/1‑138 (MQ=255)                                                                                                                                  ggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAAccc  >  2:166859/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     GGCGAACGGTCTGACCTTCGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCC  >  NZ_CP009273/3326747‑3327014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑