Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,437,182	A→G	I92T (ATC→ACC)	secY ←	preprotein translocase subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,437,182	0	A	G	100.0%	187.3 / NA	53	I92T (ATC→ACC)	secY	preprotein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/31);  total (22/31)

CCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGG  >  NZ_CP009273/3437056‑3437308                                                                                                                               |                                                                                                                               ccGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACg                                                                                                                    <  2:559112/139‑1 (MQ=255)  cGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGAGAACCACCGTCAGCAGCTGGGTAATGATCgcagc                                                                                                                   >  2:150332/1‑135 (MQ=255)   gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                  >  2:445438/1‑139 (MQ=255)    aTCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                  >  2:299774/1‑138 (MQ=255)                 ttGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCAt                                                                                                    >  1:74416/1‑139 (MQ=255)                   gCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATg                                                                                                   <  2:475403/138‑1 (MQ=255)                   gCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGa                                                                                                  >  1:475403/1‑139 (MQ=255)                      aGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATcc                                                                                               >  1:545528/1‑139 (MQ=255)                      aGCACCAGAGGACCGTAGCGGGCGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTCATGATCGACGCCGAAATATACGGCATGATcc                                                                                               >  1:404593/1‑139 (MQ=255)                       gCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATccc                                                                                              >  2:6301/1‑139 (MQ=255)                             gagTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGc                                                                                        <  1:299774/139‑1 (MQ=255)                              agTACCGTAGCGGGTGTACTGGCTGATCTTACGACGGCCAGACTCCCCTTCTTGCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCa                                                                                       <  2:34204/139‑1 (MQ=255)                                tACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCaaa                                                                                     <  1:472982/139‑1 (MQ=255)                                        cGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGCCGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAg                                                                             >  2:381363/1‑139 (MQ=255)                                        cGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAg                                                                             >  1:164339/1‑139 (MQ=255)                                        cGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAg                                                                             <  1:554958/139‑1 (MQ=255)                                        cGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAg                                                                             >  2:511836/1‑139 (MQ=255)                                        cGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAg                                                                             >  1:221456/1‑139 (MQ=255)                                          ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCTAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                           <  1:434465/139‑1 (MQ=255)                                          ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                           <  1:217635/139‑1 (MQ=255)                                          ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                           <  2:454584/139‑1 (MQ=255)                                          ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                           <  2:64133/139‑1 (MQ=255)                                             gtACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACgg                                                                        >  1:245162/1‑139 (MQ=255)                                                cTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCt                                                                      <  2:362086/138‑1 (MQ=255)                                                   gCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTga                                                                    >  2:466482/1‑137 (MQ=255)                                                   gCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTga                                                                    >  1:63719/1‑137 (MQ=255)                                                   gCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTga                                                                    >  2:21853/1‑137 (MQ=255)                                                    cTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGagag                                                                 <  1:524238/139‑1 (MQ=255)                                                       aTCTTGCGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTGGGGTGAATCATCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGctc                                                              <  2:152264/139‑3 (MQ=255)                                                       aTCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGca                                                               <  2:545528/138‑1 (MQ=255)                                                        tCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTg                                                                     >  1:442273/1‑131 (MQ=255)                                                        tCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTg                                                                     <  2:442273/131‑1 (MQ=255)                                                            acgacgACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcaccac                                                           <  2:164339/137‑1 (MQ=255)                                                                cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                     <  1:219043/139‑1 (MQ=255)                                                                cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                     <  2:355519/139‑1 (MQ=255)                                                                   ccAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACa                                                  >  2:483044/1‑139 (MQ=255)                                                                        cTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTa                                             <  1:185472/139‑1 (MQ=255)                                                                           cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCGCCACCAGAGAACATGTTaaa                                           <  1:424850/138‑1 (MQ=255)                                                                           cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                           <  2:121105/138‑1 (MQ=255)                                                                           cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                           <  1:483044/138‑1 (MQ=255)                                                                           cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaa                                            <  1:308202/137‑1 (MQ=255)                                                                                 tttcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACAtctc                                     >  2:51113/1‑138 (MQ=255)                                                                                       aaTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGAt                                                                                  <  2:342819/87‑1 (MQ=255)                                                                                       aaTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGAt                                                                                  >  1:342819/1‑87 (MQ=255)                                                                                       aaTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAatgatg                              >  2:194758/1‑139 (MQ=255)                                                                                       aaTTTCTGCCAACGTTGAGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAatgat                               <  1:381363/138‑1 (MQ=255)                                                                                        aTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAatgatg                              <  2:63719/138‑1 (MQ=255)                                                                                               ccAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                        <  1:511836/137‑1 (MQ=255)                                                                                                  aCGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTg                   <  2:221456/139‑1 (MQ=255)                                                                                                            aaCCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAg          <  1:466482/138‑1 (MQ=255)                                                                                                            aaCCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGc         <  2:14563/139‑1 (MQ=255)                                                                                                              ccaccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAg       >  1:73800/1‑139 (MQ=255)                                                                                                                   gTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTgg  <  1:276121/139‑1 (MQ=255)                                                                                                                               |                                                                                                                               CCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGG  >  NZ_CP009273/3437056‑3437308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑