Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 453,695 G→A A272T (GCG→ACG)  clpX → ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273453,6950GA96.4% 99.9 / NA 28A272T (GCG→ACG) clpXATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/15);  minor base C (1/0);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAA  >  NZ_CP009273/453571‑453829
                                                                                                                            |                                                                                                                                      
tCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCg                                                                                                                          <  1:129379/139‑1 (MQ=255)
                 tGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagcaag                                                                                                         >  1:294985/1‑139 (MQ=255)
                          aTACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGa                                                                                                <  2:285700/139‑1 (MQ=255)
                                 tAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGGTCCGGCATTGGGTTTGGGACGACGGTAAAAACGAAGTCCGACAAAGCAAGGGAAGGCGAGctgctg                                                                                         >  1:279183/1‑139 (MQ=255)
                                        cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTTCCACCGTGTAGAAACCGGCTCCGGCATTGGGTTTGGCACGGCGGTAAAAGCGAAGACCGACAAAGAAAGCGAAGGCGGGCTGCTGGCGCAgg                                                                                  >  2:478830/1‑139 (MQ=255)
                                        cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGGTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                                  >  2:482057/1‑139 (MQ=255)
                                        cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                                  >  2:5674/1‑139 (MQ=255)
                                        cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                                  <  2:1493/139‑1 (MQ=255)
                                        cTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGATAAAGTGATTTCCCCCCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                                  >  1:1493/1‑139 (MQ=255)
                                               tttGTGGCGGTGCGTTTGCCGGACTGGGTAAAGGGATTTTCCACCGTGTAGAAAACGGCTCCGGCATTGGTTTTGGGCCCACGGTAAAAGCGAAATCCGACAAAGCAAGCGAAAGCGAGCCGCCGGCGCAGGGTGAAcc                                                                           >  2:43818/1‑139 (MQ=255)
                                                  gtgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg                                                                         <  1:372366/138‑1 (MQ=255)
                                                     gCGGTGCGTTTGCCGGGCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg                                                                         >  1:37826/1‑135 (MQ=255)
                                                           cGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                               <  2:65090/139‑1 (MQ=255)
                                                                         gATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCtt                                                  <  1:450019/138‑1 (MQ=255)
                                                                              aGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                            >  1:478042/1‑139 (MQ=255)
                                                                              aGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                            <  2:478042/139‑1 (MQ=255)
                                                                                   tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg                                           <  1:559579/135‑1 (MQ=255)
                                                                                   tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg                                           >  2:559579/1‑135 (MQ=255)
                                                                                      cccACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTa                                     <  2:37826/138‑1 (MQ=255)
                                                                                             tgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgacgt                             >  1:444298/1‑139 (MQ=255)
                                                                                               tAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCt                           >  2:276813/1‑139 (MQ=255)
                                                                                                     ccGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGt                     <  1:478830/139‑1 (MQ=255)
                                                                                                     ccGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGt                     <  2:458830/139‑1 (MQ=255)
                                                                                                         cTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTc                 >  2:34179/1‑139 (MQ=255)
                                                                                                              gCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc            <  1:546875/139‑1 (MQ=255)
                                                                                                              gCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc            <  1:43818/139‑1 (MQ=255)
                                                                                                               cATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACg           <  1:34179/139‑1 (MQ=255)
                                                                                                                         ggCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa  <  1:482057/138‑1 (MQ=255)
                                                                                                                            |                                                                                                                                      
TCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAA  >  NZ_CP009273/453571‑453829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑