Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,446,256 A→C G10G (GGT→GGG)  rplC ← 50S ribosomal protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,446,2560AC100.0% 117.9 / NA 34G10G (GGT→GGG) rplC50S ribosomal protein L3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/22);  total (12/22)

TTTAGCACCGGTGGTCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTTCT  >  NZ_CP009273/3446121‑3446388
                                                                                                                                       |                                                                                                                                    
tttAGCACCGGTGGTCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATccc                                                                                                                                    <  1:52985/138‑1 (MQ=255)
              tCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGAc                                                                                                                     <  2:253679/139‑1 (MQ=255)
                     aaTAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAAc                                                                                                                <  2:317530/137‑1 (MQ=255)
                            cGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATccc                                                                                                                                    >  2:368560/1‑110 (MQ=255)
                            cGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATccc                                                                                                                                    <  1:368560/110‑1 (MQ=255)
                            cGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTg                                                                                                       >  1:453435/1‑139 (MQ=255)
                                 gCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  2:359176/138‑1 (MQ=255)
                                 gCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  1:455817/138‑1 (MQ=255)
                                    aTCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   >  1:259721/1‑135 (MQ=255)
                                    aTCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  2:259721/135‑1 (MQ=255)
                                            ccAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATc                                                                                       <  1:299940/139‑1 (MQ=255)
                                                gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAAc                                                                                                >  1:118743/1‑126 (MQ=255)
                                                gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAAc                                                                                                <  2:118743/126‑1 (MQ=255)
                                                           gAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  2:91496/112‑1 (MQ=255)
                                                           gAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   >  1:91496/1‑112 (MQ=255)
                                                           gAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        >  1:444035/1‑139 (MQ=255)
                                                            aGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGa                                                                         <  1:581011/137‑1 (MQ=255)
                                                            aGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGa                                                                         <  1:207994/137‑1 (MQ=255)
                                                            aGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGa                                                                         <  1:260279/137‑1 (MQ=255)
                                                            aGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        <  1:498869/138‑1 (MQ=255)
                                                                aCGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAc                                                                    <  2:453435/138‑1 (MQ=255)
                                                                 cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             >  2:403402/1‑128 (MQ=255)
                                                                 cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             <  1:403402/128‑1 (MQ=255)
                                                                             cAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  1:100256/138‑1 (MQ=255)
                                                                             cAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  2:476589/138‑1 (MQ=255)
                                                                               aCTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCAc                                                    <  1:180554/139‑1 (MQ=255)
                                                                                     aTTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATc                                                                                       >  2:520848/1‑98 (MQ=255)
                                                                                     aTTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATc                                                                                       <  1:520848/98‑1 (MQ=255)
                                                                                               cTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGcc                                    <  1:458743/139‑1 (MQ=255)
                                                                                                    aTAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  1:434212/115‑1 (MQ=255)
                                                                                                    aTAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       >  2:434212/1‑115 (MQ=255)
                                                                                                        aaaCGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGa                           >  2:71189/1‑139 (MQ=255)
                                                                                                          aCGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             >  1:567740/1‑87 (MQ=255)
                                                                                                          aCGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             <  2:567740/87‑1 (MQ=255)
                                                                                                                          aGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACg         <  1:577677/139‑1 (MQ=255)
                                                                                                                                 ggTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTtct  >  1:342567/1‑139 (MQ=255)
                                                                                                                                 ggTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTtct  >  2:212676/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
TTTAGCACCGGTGGTCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTTCT  >  NZ_CP009273/3446121‑3446388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑